摘要

      本研究探讨了枯草芽孢杆在堆肥过程中菌以4个接种比例（0, 0.5%, 1%, and 2% w/w 堆肥）对ARGs、可移动基因元件和细菌群落变化的影响。堆肥过程对ARGs的影响大于枯草芽孢杆菌。检测到的主要ARG是sul1。枯草芽孢杆菌添加量为0.5%时，成熟产品中ARGs、MGEs和人类致病菌的相对丰度降低了2-3logs。网络分析和冗余分析表明，intI1、Firmicutes和pH是ARGs变化的主要原因，控制这些因素可能有助于抑制ARGs的传播。

      This study explored the effects of Bacillus subtilis at four levels (0, 0.5%, 1%, and 2% w/w compost) on the variations in ARGs, mobile genetic elements (MGEs), and the bacterial community during composting. The composting process had a greater impact on ARGs than Bacillus subtilis. The main ARG detected was sul1. The addition of Bacillus subtilis at 0.5% reduced the relative abundances of ARGs, MGEs, and human pathogenic bacteria (by 2-3 logs) in the mature products. Network and redundancy analyses suggested that intI1, Firmicutes, and pH were mainly responsible for the changes in ARGs, thus controlling these factors might help to inhibit the spread of ARGs.

       https://www.sciencedirect.com/science/article/abs/pii/S0960852419312416?via%3Dihub