摘要

       在农田土壤中的土壤动物内脏中发现了多种ARGs。生物炭已广泛应用于农田土壤修复和改善土壤质量，但其对土壤动物肠道相关ARG的影响尚不清楚。在本研究中，我们采用六种不同的生物沙土改良土壤，培育出弹尾虫。采用高通量qPCR技术建立了弹尾类动物内脏及周围土壤的ARG剖面。在弹尾目动物内脏和土壤中分别检测到73种和162种ARGs亚型。生物炭改性显著改变了弹尾目动物内脏和土壤中的ARG组成，且呈生物炭质量依赖性。然而，只有含有高浓度重金属的有机肥生物炭才能增加肠道相关arg的相对丰度。肠道微生物群落、MGEs和生物炭性质的变化解释了弹尾目动物肠道总ARG变化的84%。本研究结果提示，生物炭的特性应受到重视，因为生物炭中高剂量的重金属会增加弹尾目内脏中ARGs的丰度，从而通过弹尾目运动促进ARGs在环境中的扩散。

        A diverse array of ARGs has been detected in the guts of soil fauna residing in farmland soil. Biochar has been widely used in farmland for soil remediation and improvement of soil quality; however, the effects of biochar amendment on the gut-associated ARGs of soil fauna remain unclear. In the present study, collembolans were cultivated in soils amended with 6 types of biochars. High-throughput qPCR was used to establish ARG profiles of the collembolan guts as well as the surrounding soils. A total of 73 and 162 subtypes of ARGs were detected in the collembolan guts and soils, respectively. Biochar amendment significantly altered the ARG compositions of the collembolan guts and soils, in a biochar quality-dependent manner. However, only manure-derived biochar, which contained elevated concentrations of heavy metals, increased the relative abundance of gut-associated ARGs. Changes in the gut microbial community, MGEs and biochar properties explained 84% of the total ARG variations in the collembolan guts. The findings of this study suggested that biochar properties should receive more attention, as high doses of heavy metals in biochar could increase the abundance of ARGs in collembolan guts, thereby contributing to the spread of ARGs in the environment through collembolan movement.

         https://www.sciencedirect.com/science/article/abs/pii/S0304389419306338?via%3Dihub