摘要

        抗生素耐药基因（ARGs）的流行和扩散已被认为是一种新出现的令人关注的污染物，是对全球公共卫生的重大威胁。为了确定人工农业灌溉系统中ARGs的发生和分布，我们在内蒙古河套灌区设计了8个农田排水样地。结果表明，地下排水渠中ARGs的分布受当地城镇、农牧业结构的影响。blaTEM基因在水样中占优势（高达8.98个ARG拷贝/16srRNA基因）。排水渠采样点的平均ARG丰度明显高于黄河来水，说明人工农业灌溉系统提高了研究区的抗性基因丰度。此外，整个灌溉系统的出水中ARGs的丰度比进水低。这说明五粮素海流域生态系统在调节该区ARGs丰度方面具有重要作用。在我们的研究中，在灌溉系统中，与trB、emrD、mexF和vanC（P < 0.001）相关的流动基因元素。此外，ARGs的其他特殊亚型之间也存在着不同的相关性。这些发现为减少ARGs的传播和相关的公共健康风险提供了更深入的见解。

        The prevalence and proliferation of antibiotic resistance genes (ARGs) has been identified as an emerging contaminant of concern and a crucial threat to public health worldwide. To determine the occurrence and distribution of ARGs in artificial agricultural irrigation systems, we designed eight sample sites of farmland drainage in the Hetao Irrigation District, Inner Mongolia, China. Results indicated that the distribution of ARGs in sub-drainage canals is influenced by the local urban area, agriculture, and animal husbandry structure. The bla_TEM gene was predominant in the water samples (up to 8.98 ARG copies/16S rRNA genes). The average ARG abundance in drainage channel sampling sites was significantly higher than the influent water from the Yellow River, which means that the artificial agricultural irrigation system enhances the abundance of resistance genes in the study area. Moreover, the effluent water of the whole irrigation system presented a lower abundance of ARGs than the influent water. This demonstrates that the Wuliangsuhai watershed ecosystem plays an important role in regulating the abundance of ARGs in the area. In our study, the mobile gene elements correlated with trB, emrD, mexF, and vanC (P < 0.001) in the irrigation system. Additionally, different correlations exist between other special subtypes of ARGs. These findings provided deeper insights into mitigating the propagation of ARGs and the associated risks to public health.

         https://www.sciencedirect.com/science/article/pii/S0269749118357129?via%3Dihub