摘要

      水传抗生素耐药基因（ARGs）的广泛存在对数百万人的健康构成了日益严重的威胁。本研究检测了上海河网10个ARG亚型的相对丰度，其中主要的ARG组分为strB、sul1和ermB。综合污水、城市污水处理厂尾水和农业区径流，分别到达苏州河（SZ）、甸浦河（DP）和黄浦江（HP），显著富集了这些arg（单因素方差分析，P<0.01）。目标arg在不同的河流中以不同的模式分布。blaCTX-M和blaTEM有助于受城市源影响的河流中总ARGs的增加，特别是SZ河，其ARGs的分布与整个河网汇流的分布显著相关（Mantel检验，P <8239; 0.01）。细菌群落结构与ARGs密切相关，下游样本中潜在致病菌与目标ARGs的相关性显著（Procrustes检验，P = 0.03）。在DP河中，城市污水沉降物附近的水体中intl1含量最高，其下游样本的intl-ARG关系符合网络汇流的回归模型（R = 0.84，P <0.001）。河流水质的改善并不能降低ARGs，但可能影响ARGs在上海河网中的分布格局。

       The widespread of water borne antibiotic resistance genes (ARGs) represents a growing threat to the health of millions of people. Our study detected the relative abundances of 10 ARG subtypes in the Shanghai river network, where the major ARG components were strB, sul1, and ermB. These ARGs were significantly enriched by the combined sewage, tail water from urban wastewater treatment plant and runoff from agricultural areas, which reached the Suzhou (SZ), Dianpu (DP), and Huangpu (HP) River, respectively (one-way ANOVA, P < 0.01). The target ARGs were distributed in varying patterns across different rivers. bla_CTX-M and bla_TEM contributed to the increase of total ARGs in the rivers influenced by urban sources, particularly in the SZ River, whose distribution of ARGs was significantly related to that of the confluence of the whole river network (Mantel test, P < 0.01). The bacterial community was closely structured with ARGs and potential pathogenic bacteria's association with target ARGs became significant in downstream samples (Procrustes test, P = 0.03). Water near urban wastewater fallouts was observed to have the highest content of intl1 in the DP River, whose downstream samples' intl -ARG relationship fitted the same regression model as that of the network confluence (R = 0.84, P < 0.001). The amelioration of river water quality does not reduce ARGs, but may affect their distributional patterns in the river network in Shanghai.

       https://www.sciencedirect.com/science/article/abs/pii/S0043135419302234?via%3Dihub