摘要

       有机农产品近年来发展迅速。然而，在有机农业中引入更多的抗生素抗性基因（ARGs）和畜禽粪便所带来的潜在食品安全风险一直被忽视。本研究分别对有机鱼腥草、化学鱼腥草和野生鱼腥草中的细菌群落、22种四环素、3种氨基糖苷和4种β-内酰胺ARGs进行了研究。共检测到9种四环素、3种氨基糖苷和2种β-内酰胺ARG亚型，有机HCT中ARG亚型较多。有机六氯环己烷中总ARGs的绝对和相对丰度显著高于化学六氯环己烷和野生六氯环己烷。肠杆菌科、气单胞菌科、假单胞菌科、冰碛菌科和草酸杆菌科是化学和野生HCT的优势类群，而有机HCT中，只有肠杆菌科占细菌群落的83.23%-87.40%。14个细菌家族可能是HCT中ARG亚型的宿主。肠杆菌科是一个可能的宿主菌亚型，包括tetA、tetB、tetC、tetE和aadA，是影响ARGs在HCT中行为的主要细菌。此外，四环素ARG亚型有更多可能的宿主。这些结果有助于更好地了解ARG在食品安全中的潜在风险，并制定有效的措施防止ARG在有机农产品中的传播。

        The organic agricultural products has been growing rapidly in recent years. However, a potential food safe risk, resulted by introduction more antibiotic resistant genes (ARGs) accompanied with animal manure using to organic farming, has long been overlooked. In current study, the bacterial community, 22 tetracycline, 3 aminoglycoside and 4 β-lactams ARGs were respectively investigated in the organic, chemical and wild Houttuynia cordata Thunb (HCT). A total of 9 tetracycline, 3 aminoglycoside and 2 β-lactam ARG subtypes were detected, and the organic HCT harbored more ARG subtypes. The absolute and relative abundance of total ARGs in organic HCT was strikingly higher than that in chemical and wild HCT. The Enterobacteriaceae, Aeromonadaceae, Pseudomonadceae, Moraxellaceae and Oxalobacteraceae were the dominant taxa in the chemical and wild HCT, but in the organic HCT, only Enterobacteriaceae posed 83.23% - 87.40% of bacterial community. Fourteen bacterial families might be the possible hosts of ARG subtypes in the HCT. Enterobacteriaceae was a possible host of most ARG subtypes, including tetA, tetB, tetC, tetE and aadA, and it was the main bacteria affecting the behavior of ARGs in the HCT. Additionally, the tetracycline ARG subtypes had more possible hosts. These results help to better understand the ARG potential food safe risk and develop effective measures to prevent the ARG dissemination in organic agricultural product.

        https://www.sciencedirect.com/science/article/abs/pii/S0963996918309177?via%3Dihub