摘要

      采用四环素类药物和磺胺类药物连续搅拌槽式消化器，研究了抗生素压力对污泥厌氧消化的影响。在抗生素压力下，多功能产甲烷菌和严格氢化产甲烷菌分别增加和减少21.1%和10.9%。KEGG分析表明，氢氧化和乙酰化产甲烷途径均受到影响。与脂质代谢、碳水化合物代谢和脂肪酸降解有关的功能基因丰度的减少，将导致甲烷产量减少25%。网络分析表明，四环素残留量、抗性基因（ARGs）丰度和潜在寄主的特定成员之间存在正相关。污泥中广泛检测到1000多种ARG亚型，包括大环内酯类（28%）、四环素类（24%）、氟喹诺酮类（20%）和多肽类（20%）耐药基因。长期接触抗生素的AD过程会增加ARG的多样性和丰度，增强ARG与特定微生物的联系，筛选出具有趋化机制的细菌。

       Continuous stirred-tank digesters with tetracyclines and sulfonamides were operated to investigate the impacts of antibiotic pressure on sludge anaerobic digestion. The versatile methanogen Methanosarcinales and strictly hydrogenotrophic methanogen Methanobacteriales increased and decreased by 21.1% and 10.9% under antibiotic pressure, respectively. KEGG analysis revealed that hydrogenotrophic and acetoclastic methanogenesis pathways were all affected. The decrease in abundance of function genes involved in lipid metabolism, carbohydrate metabolism, and fatty acid degradation, would lead to a reduction in methane production by 25%. Network analysis indicated positive associations among tetracycline residuals, abundance of resistance genes (ARGs), and specific member of potential hosts. Over 1000 ARG subtypes were widely detected in sludge, including macrolide (28%), tetracycline (24%), fluoroquinolone (20%), and peptide (20%) resistance genes. AD process exposed to long-term antibiotic would increase the diversity and abundance of ARG, enhance the association of ARG with specific microbes, and select bacteria able to perform chemotaxis mechanism.

        https://www.sciencedirect.com/science/article/abs/pii/S0960852419303281?via%3Dihub