摘要

      了解堆肥过程中影响抗生素抗性基因（ARGs）传播的主要驱动因素，对去除ARGs具有重要意义。在本研究中，将三种浓度的泰乐菌素（25、50和75 mg kg-1，以干重为基础）添加到猪粪中，再加上一个对照组，用棉秆堆肥。每种治疗方法重复三次，用不同浓度的泰乐菌素测定ARGs分布。根据堆肥时间，将前35个属和ARGs分布聚在一起。组合堆肥参数（温度、pH、NH4+-N、NO3-N和水分含量）占潜在宿主菌变化总量的78.4%。此外，根据冗余分析，选择的5个堆肥参数和6个菌门（包括25个潜在的寄主菌属）分别解释了ARG谱的46.9%和30.7%的变异。堆肥过程中ARGs的变化主要受潜在寄主菌的动态而非整合子的影响，以及生物铜和生物锌的选择压力的影响。

       Understanding the main drivers that affect the spread of antibiotic resistance genes (ARGs) during the composting process is important for the removal of ARGs. In this study, three levels of tylosin (25, 50, and 75 mg kg^-1 on a dry weight basis) were added to swine manure plus a control, which was composted with cotton stalks. Each treatment was repeated in triplicate and the ARG profiles were determined with different levels of tylosin. The top 35 genera and ARGs profiles were clustered together based on the composting time. Combined composting parameters (temperature, pH, NH₄⁺-N, NO₃-N, and moisture content) accounted for 78.4% of the total variation in the changes in the potential host bacteria. In addition, the selected five composting parameters and six phyla (including 25 potential host bacterial genera) explained 46.9% and 30.7% of the variation in the ARG profiles according to redundancy analysis, respectively. The variations in ARGs during the composting process were mainly affected by the dynamics of potential host bacteria rather than integrons and the selective pressure due to bio-Cu and bio-Zn.

       https://www.sciencedirect.com/science/article/abs/pii/S0304389418304928?via%3Dihub