摘要

       抗生素抗性基因（ARGs）、人类病原菌（HPB）和抗生素抗性HPB（ARHPB）从动物饲养场向周围环境传播，对环境生态和公众健康构成潜在的高风险。在这里，一个亚基因组分析被用来探索ARGs，HPB和ARHPB从猪饲养场传播到周围的河流和农业土壤。共检出抗生素残留（ND）-1628.4μg/kg，18种ARGs，48种HPB，216株ARB。猪场抗生素残留主要迁移到河流沉积物和温室土壤中。猪饲养场的优势ARGs和HPB物种扩散到河流沉积物（四环素抗性基因、艰难梭菌和结核分枝杆菌）、溪水（多药抗性（MDR）基因、福氏志贺菌和百日咳杆菌）和温室土壤（MDR基因、炭疽杆菌和布鲁氏菌）。所有标本216株ARB中54.4%为潜在ARHPB种，4株MDR-HPB的基因组测序和功能注释显示有9种ARG型。我们的发现揭示了抗生素残留、ARGs、HPB和ARHPB通过猪污水排放和肥料施肥从猪饲养场向周围河流和农业土壤的潜在迁移和传播。

        The dissemination of antibiotic resistance genes (ARGs), human pathogenic bacteria (HPB), and antibiotic-resistant HPB (ARHPB) from animal feedlot to nearby environment poses a potentially high risk to environmental ecology and public health. Here, a metagenomic analysis was employed to explore the dissemination of ARGs, HPB, and ARHPB from a pig feedlot to surrounding stream and agricultural soils. In total, not detectable (ND)-1,628.4 μg/kg of antibiotic residues, 18 types of ARGs, 48 HPB species, and 216 ARB isolates were detected in all samples. Antibiotic residues from pig feedlot mainly migrated into stream sediments and greenhouse soil. The dominant ARGs and HPB species from pig feedlot spread into stream sediments (tetracycline resistance genes, Clostridium difficile, and Mycobacterium tuberculosis), stream water (multidrug resistance (MDR) genes, Shigella flexneri, and Bordetella pertussis), and greenhouse soil (MDR genes, Bacillus anthracis, and Brucella melitensis). It is concerning that 54.4% of 216 ARB isolates from all samples were potential ARHPB species, and genome sequencing and functional annotation of 4 MDR HPB isolates showed 9 ARG types. Our findings revealed the potential migration and dissemination of antibiotic residues, ARGs, HPB, and ARHPB from pig feedlot to surrounding stream and agricultural soils via pig sewage discharge and manure fertilization.

        https://www.sciencedirect.com/science/article/abs/pii/S0304389418304205?via%3Dihub