摘要

        研究了6个城市污水处理厂（WWTPs）处理城市和工业废水中抗生素耐药基因（ARGs）的数量变化。在选定的污水处理厂中，研究了ARGs在1年时间间隔和污水处理过程的两个阶段中的去向。共测定了9个ARG（tetW、tetO、tetA、tetB、tetM、blaTEM、ermB、sul1和intl1），并用ARG拷贝数/16SrRNA拷贝数评估了它们的相对丰度。从四环素抗性基因来看，tetW是所有样本污水处理厂中唯一检测到的基因。第五污水处理厂硝化池1年内相对丰度稳定，经二沉处理后相对丰度较硝化池降低24%。显示出高四环素抗性（最小抑制浓度>100μg/mL）的菌株被鉴定为不动杆菌属、克雷伯菌属、柠檬酸杆菌属、芽孢杆菌属和肠杆菌属。第五污水处理厂废水中ermB和sul1的相对丰度也发生了动态变化。

         Quantitative changes in antibiotic resistance genes (ARGs) were investigated in six urban wastewater treatment plants (WWTPs) treating municipal and industrial wastewaters. In a selected WWTP, the fate of ARGs was studied in a 1-year time interval and in two phases of wastewater treatment process. Nine ARGs (tetW, tetO, tetA, tetB, tetM, bla_TEM, ermB, sul1, and intl1) were quantified in total and their relative abundance assessed by ARG copies/16SrRNA copies. From the tetracycline resistance genes, tetW was the only one detected in all sampled WWTPs. Its relative abundance in the nitrification tank of WWTP5 was found stable during the 1-year period, but was lowered by secondary sedimentation processes in the wastewater treatment down to 24% compared to the nitrification tank. Bacterial isolates showing high tetracycline resistance (minimal inhibition concentrations >100 μg/mL) were identified as members of Acinetobacter, Klebsiella, Citrobacter, Bacillus, and Enterobacter genera. Dynamic shifts in the relative abundance of ermB and sul1 were also demonstrated in wastewater samples from WWTP5.

          https://www.ncbi.nlm.nih.gov/pubmed/29847746