摘要

       采用生物膜电极反应器（BER）系统，构建了一个连续流动的微生物燃料电池人工湿地（MFC-CW）去除磺胺甲恶唑（SMX）。以多层MFC-CWs为动力的BER单元作为预处理单元，出水进入MFC-CW进一步降解。实验结果表明，在BER装置中，2或4 mg/L SMX的去除率接近90%，在耦合系统中的总去除率超过99%。随着水力停留时间（HRT）从16 h降低到4 h，误码率中的SMX去除率从75%下降到48%。但耦合系统的总去除率仍在97%以上。MFC-CW中的最大SMX去除率为阳极层，占总去除率的42%-55%。此外，系统中sul基因的相对丰度依次为sulI > sulII > sulII > sulII，sul基因拷贝数与SMX浓度和16srrna基因拷贝数呈显著正相关。此外，sul基因、16srrna基因拷贝数与HRT呈显著负相关。MFC-CW出水中sul基因的丰度低于BER出水中的丰度。高通量测序结果表明，运行时间、供电方式和HRT对BER的微生物群落多样性有影响。MFC-CW产生的生物电能可能降低微生物群落多样性，并有助于降低BER中的抗生素抗性基因（ARG）丰度。综上所述，BER-MFC-CW耦合系统是处理含SMX废水和降低相应精氨酸丰度的潜在工具。

        A continuous flow microbial fuel cell constructed wetland (MFC-CW) coupled with a biofilm electrode reactor (BER) system was constructed to remove sulfamethoxazole (SMX). The BER unit powered by the stacked MFC-CWs was used as a pretreatment unit, and effluent flowed into the MFC-CW for further degradation. The experimental results indicated that the removal rate of 2 or 4 mg/L SMX in a BER unit was nearly 90%, and the total removal rate in the coupled system was over 99%. As the hydraulic retention time (HRT) was reduced from 16 h to 4 h, the SMX removal rate in the BER decreased from 75% to 48%. However, the total removal rate in the coupled system was still over 97%. The maximum SMX removal rate in the MFC-CW, which accounted for 42%-55% of the total removal, was obtained in the anode layer. In addition, the relative abundances of sul genes detected in the systems were in the order of sulI > sulII > sulIII, and significant positive correlations of sul gene copy numbers versus SMX concentration and 16S rRNA gene copy numbers were observed. Furthermore, significant negative correlations were identified between sul genes, 16S rRNA gene copy numbers, and HRT. The abundances of the sul genes in the effluent of the MFC-CW were lower than the abundances observed in the BER effluent. High-throughput sequencing revealed that the microbial community diversity of the BER was affected by running time, power supply forms and HRT. Bio-electricity from the MFC-CW may reduce microbial community diversity and contribute to reduction of the antibiotic resistance gene (ARG) abundance in the BER. Taken together, the BER-MFC-CW coupled system is a potential tool to treat wastewater containing SMX and attenuate corresponding ARG abundance.

       https://www.sciencedirect.com/science/article/pii/S0048969718315432?via%3Dihub