摘要

      污水处理厂（WWTPs）含有多种抗生素抗性基因（ARGs），因此被认为是这些基因向环境中传播的主要途径。然而，对废水处理过程中ARGs动态的综合评价缺乏对ARGs的广泛研究。本文采用高通量定量PCR和基于16srRNA的Illumina测序技术，对11个中国污水处理厂的114个样品进行了ARGs和细菌群落结构的动态研究。结果表明，在去除1-2个数量级ARGs浓度的情况下，ARGs剖面发生显著变化，废水处理工艺可以显著降低ARGs的丰度和多样性。然而，与进水相比，废水中检测到大量的ARGs并富集。特别是废水处理后的污水处理厂样品中，有7种arg主要表现为对β-内酰胺类和氨基糖苷类的抗性，还有3种可移动的遗传元素。不同的污水处理工艺、不同的季节、不同的地区，ARGs的分布也不同。本研究追踪污水处理过程中ARGs的足迹，为评估污水处理厂ARGs的扩散提供依据，并为确定改善污水处理厂ARG缓解的管理方案提供数据。

       Wastewater treatment plants (WWTPs) contain diverse antibiotic resistance genes (ARGs), and thus are considered as a major pathway for the dissemination of these genes into the environments. However, comprehensive evaluations of ARGs dynamic during wastewater treatment process lack extensive investigations on a broad spectrum of ARGs. Here, we investigated the dynamics of ARGs and bacterial community structures in 114 samples from eleven Chinese WWTPs using high-throughput quantitative PCR and 16S rRNA-based Illumina sequencing analysis. Significant shift of ARGs profiles was observed and wastewater treatment process could significantly reduce the abundance and diversity of ARGs, with the removal of ARGs concentration by 1-2 orders of magnitude. Whereas, a considerable number of ARGs were detected and enriched in effluents compared with influents. In particular, seven ARGs mainly conferring resistance to beta-lactams and aminoglycosides and three mobile genetic elements persisted in all WWTPs samples after wastewater treatment. ARGs profiles varied with wastewater treatment processes, seasons and regions. This study tracked the footprint of ARGs during wastewater treatment process, which would support the assessment on the spread of ARGs from WWTPs and provide data for identifying management options to improve ARG mitigation in WWTPs.

        https://www.sciencedirect.com/science/article/pii/S0160412017322079?via%3Dihub