摘要

    城市固体废物的有机部分（OFMSW）已成为抗生素抗性基因（ARG）的来源和储库之一。探索OFMSW生物处理过程中ARG的命运至关重要。因此，通过定量PCR分析了厌氧消化OFMSW期间几种类型的ARGs和整合子基因的变化。此外，研究了不同粒径的活性炭对靶基因行为的影响以及ARGs动力学的潜在微生物机制。结果表明，无论有无活性炭存在，厌氧消化后初始系统中的总ARGs均降低。总ARGs绝对丰度的去除率为29.95％-63.40％。在最终的OFMSW厌氧消化系统中，粉状活性炭（PAC）组的总ARGs含量显着高于对照组（P <0.05）。 PAC的添加抑制了ARGs的减少，而颗粒状活性炭的添加对ARGs的变化没有显着影响。在厌氧消化过程中，ARGs的潜在宿主细菌主要是梭菌，细菌和增效菌。添加PAC引起的宿主细菌富集是靶基因增加的主要原因。而且，梭状芽胞杆菌可能已经是导致ARGs增长和下降的主要驱动因素。这些结果将有助于我们了解OFMSW厌氧消化过程中ARG的分布以及活性炭添加对ARG的影响。

    The organic fraction of municipal solid waste (OFMSW) has become one of the sources and reservoirs of antibiotic resistance genes (ARGs). It is essential to explore the fate of ARGs during biological treatment of OFMSW. Therefore, the changes in several types of ARGs and integron genes during anaerobic digestion of the OFMSW were analyzed by quantitative PCR. Furthermore, the effects of different particle sizes of activated carbon on the behaviors of the target genes and the potential microbial mechanisms of ARGs dynamics were investigated. The results showed that the total ARGs in the initial system were reduced after anaerobic digestion with or without the presence of activated carbon. The removal rate of the absolute abundance of total ARGs was 29.95%-63.40%. In the final system of anaerobic digestion of the OFMSW, the abundance of total ARGs in powdered activated carbon (PAC) groups was significantly higher than that in the control group (P<0.05). The supplementation of PAC inhibited the reduction of ARGs, and the supplementation of granular activated carbon had no significant effect on the change in ARGs. The potential host bacteria of ARGs were mainly Clostridia, Bacteroidia, and Synergistia during anaerobic digestion. The enrichment of host bacteria caused by PAC addition was the main reason for the increase in the target genes. Moreover, Clostridia might have been the main driving factor for the growth and decline of ARGs. These results will help us to understand the dissemination of ARGs and the impacts of activated carbon addition on ARGs during anaerobic digestion of the OFMSW.

    https://europepmc.org/article/med/33884812