摘要

    在生活世界的所有可能视域中，不良的抗生素耐药性的恶性传播引起了人们的极大关注，需要立即予以关注。由于我们担心这些抗生素抗性基因（ARG）的普遍性，因此研究表明在高度控制的环境（如新生儿重症监护病房（ICU））中存在抗生素抗性决定簇。典型的新生儿设施中存在ARGs是现代的噩梦。在目前的工作中，通过宏基因组学方法分析了在ICU照料下的婴儿的新生儿肠道抵抗组，以检查ARGs在新生儿照护单位中传播的可能性。发现所有样品均富含ARG，每个样品含有176至291个ARG，每份16S rRNA基因的含量为7.68至12.86份ARG。在所有ARG中，氨基香豆素（mdtA，mdtC），氨基糖苷（cpxA，APH（3''）-Ib）和杆菌肽（BacA）含量最高。分析还发现，与质粒ARGs相比，染色体ARGs的丰度明显更高（p <0.05）。我们还发现，与移动遗传元件相比，ARGS与金属抗性基因高度相关。此外，基于外排泵的毒力因子基因也与ARGs高度相关。同时，带有重叠群的ARGs的分类学显示了大部分Klebsiella和Enterobacter属。本研究表明，新生儿重症监护病房中较高的肠道抵抗力可能是由于新生儿病房的无菌条件受损，即使在受控环境下，新生儿的患病风险也更大。

    The vicious spread of undesired antibiotic resistance among all the possible horizons of the living world is a cause of great concern and requires immediate attention. As we are concerned about the prevalence of these antibiotic resistance genes (ARGs), studies have suggested the presence of antibiotic resistant determinants in highly controlled environments such as neonatal-intensive care units (ICU). The presence of ARGs in the typical neonatal facilities are a kind of modern nightmare. In the present work, neonatal gut resistome from infants under ICU care was analyzed by metagenomic approach, to examine the possibility of spread of ARGs in neonatal care units. All samples were found to be rich in ARGs and were containing 176 to 291 ARGs per sample and the abundance ranging from 7.68 to 12.86 copies of ARGs per copy of the 16S rRNA gene. Among the all ARGs, Aminocoumarins (mdtA, mdtC), Aminoglycoside (cpxA, APH(3'')-Ib) and Bacitracin (BacA) were the most abundant. The analysis also found that chromosomal ARGs were having significantly higher abundance compared to plasmid ARGs (p < 0.05). We also found that ARGS were highly correlated to metal resistant genes, as compares to mobile genetic elements. Moreover, Efflux pump-based virulence factor genes were also highly associated with ARGs. While, the taxonomy of ARGs carrying contigs showed the majority of genera Klebsiella and Enterobacter. The present study showed, that the higher gut resistome in neonatal ICUs could be due to the compromised sterile conditions in the neonatal units, which present a greater risk to the neonates even in the controlled environment.

    https://sciforum.net/paper/view/9650