摘要

    中国的废水处理厂（WWTP）已通过各种新兴工艺进行了升级或改造，但仍缺乏对这些废水处理厂中抗生素抗性基因（ARG）行为的全面了解。在这里，在具有升级过程的废水处理厂（WWTP-UP）中研究了ARGs的分布和细菌群落。在所有样品中检测到238个独特的ARG。在研究期间，整个治疗过程中平均ARGs浓度降低了98.4％。 A2 / O膜生物反应器（MBR）的去除效率明显高于A2 / O高效絮凝沉降/布介质过滤器（HEFS / CMF）的去除效率（p <0.05），分别为3.5和2.1平均分别记录日志值。值得注意的是，所有样品中均存在35个ARG和14个移动遗传元件（MGE）。根据网络分析显示的共现模式，持久性ARG可能通过持久性MGE在持久性细菌之间的转移而传播。使用多元线性回归分析，我们获得了3至5种主要ARG类型的可能指标，这些指标可用于评估ARG的总体分布甚至预测不同ARG类型的丰度。我们的发现提供了有关升级过程对ARG的影响的新见解，并强调了需要更好的策略来改善污水处理厂中ARG消除的需求。

    Wastewater treatment plants (WWTPs) in China have been upgraded or renovated with a variety of emerging processes, but a comprehensive understanding of the behavior of antibiotic resistance genes (ARGs) in these WWTPs is still lacking. Here, the distribution of ARGs and bacterial community were investigated in a wastewater treatment plant with upgrading processes (WWTP-UP). 238 unique ARGs were detected in all samples. During the study period, the average ARGs concentration decreased by 98.4% along the entire treatment process. The removal efficiency of A2/O-membrane bioreactor (MBR) process was significantly higher than that of A2/O-high efficiency flocculent settling/cloth media filter (HEFS/CMF) process (p < 0.05), which corresponded to 3.5 and 2.1 log values on average, respectively. Notably, 35 ARGs and 14 mobile genetic elements (MGEs) were persistent in all samples. Based on the co-occurrence pattern revealed by network analysis, persistent ARGs possibly spread through the transfer of persistent MGEs among persistent bacteria. Using multiple linear regression analysis, we obtained 3 to 5 possible indicators for major ARG types, which might be served to evaluate the general distribution of ARGs or even predict the abundance of different ARG types. Our findings provide new insights into the impacts of upgrading process on ARGs and highlight the need for better strategies to improve ARGs elimination in WWTPs.

    https://www.sciencedirect.com/science/article/abs/pii/S0048969720383479