摘要

    抗生素抗性基因（ARGs）作为新兴污染物已在水环境中广泛传播。野生鱼类可能是水环境中ARGs的接受者和传播者，但是，很少有人报道野生鱼类和相关水环境中ARGs的分布和传播。在这里，我们使用高通量qPCR和16S rRNA基因序列分析了郊区淡水鱼和相关水域中野生淡水鱼和相关水中的ARG和细菌群落。鱼和水样品中总共鉴定出80和220种独特的ARG亚型。鱼和水均显示出显着的ARG季节变化（P <0.05）。鱼和水中ARGs的绝对丰度最高，分别发生在夏季（平均每克1.32×109份）和秋季（每毫升9.04×106份）。此外，二分网络分析表明，鱼和水中连续存在9种ARG和1种移动遗传元素。此外，发现鱼类和水中共享的细菌与碎片ARGs显着相关。研究结果表明，鱼和水中的细菌和ARGs可以相互连接，并且ARGs可能会以细菌为传播介质在鱼和水之间转移。

    Antibiotic resistance genes (ARGs) as emergence contaminations have spread widely in the water environment. Wild fish may be recipients and communicators of ARGs in the water environment, however, the distribution and transmission of ARGs in the wild fish and relevant water environment were rarely reported. Here, we have profiled ARGs and bacterial communities in wild freshwater fish and relevant water in a peri-urban river using high-throughput qPCR and 16S rRNA gene sequence. A total of 80 and 220 unique ARG subtypes were identified in fish and water samples. Fish and water both showed significant ARG seasonal variations (P < 0.05). The highest absolute abundance of ARGs in fish and water occurred in summer (1.32 × 109 copies per g, on average) and autumn (9.04 × 106 copies per mL), respectively. In addition, the bipartite network analysis showed that 9 ARGs and 1 mobile genetic element continuously shared in fish and water. Furthermore, bacteria shared in fish and water were found to significantly correlate with shard ARGs. The findings demonstrate that bacteria and ARGs in fish and water could interconnect and ARGs might transfer between fish and water using bacteria as a spreading medium.

    https://www.sciencedirect.com/science/article/abs/pii/S1001074220304290