摘要

    抗生素抗性基因（ARGs）作为一种新兴的环境污染物已经引起了广泛的关注，这些污染物可能威胁到人类。猪场废水是ARGs的主要排放源之一。在这项研究中，进行了包括超滤和两级反渗透在内的综合膜过滤，以处理猪废水。根据宏基因组测序，在膜过滤过程的每个阶段，通过定量实时PCR（qPCR）对16种目标ARG的丰度进行了定量分析，这些含量占猪废水中总ARG的72.64％。结果表明，集成膜过滤可减少99.0％以上的常规污染物和99.79％的ARG（从3.02×108拷贝数/ mL降至6.45×105拷贝数/ mL）。主成分分析（PCA）表明，膜过滤对ARGs亚型的去除效率与ARGs类型无关。但是，根据共生模式，ARG与废水质量指标TP，SS和EC之间存在很强的相关性，这表明ARG的去除与猪废水中的不溶性固体颗粒和可溶性离子密切相关。这些结果表明，膜过滤不仅可以去除氮，磷等常规污染物，而且可以减少ARGs的新兴污染物，降低ARGs流入天然水的风险。

    Antibiotic resistance genes (ARGs) have attracted extensive attention as an emerging environmental contaminant potentially threatening humans. One of the main emission sources of ARGs is swine wastewater. In this study, integrated membrane filtration including ultrafiltration and two-stage reverse osmosis was conducted for swine wastewater treatment. The abundances of 16 target ARGs, which accounted for 72.64% of the total ARGs in swine wastewater according to metagenomic sequencing, were quantified by quantitative real-time PCR (qPCR) during each stage of the membrane filtration process. The results showed that integrated membrane filtration could reduce more than 99.0% of conventional pollutants and 99.79% of ARGs (from 3.02 × 108 copy numbers/mL to 6.45 × 105 copy numbers/mL). Principal component analysis (PCA) indicated that the removal efficiency of ARGs subtype by membrane filtration did not depend on ARGs type. However, strong correlations were found between ARGs and the wastewater quality indicators TP, SS and EC according to Cooccurrence patterns, indicating that ARG removal was closely associated with insoluble solid particles and soluble ions in swine wastewater. These results showed that membrane filtration could not only remove conventional pollutants such as nitrogen and phosphorus but also reduce the emerging pollutant of ARGs and decrease the risk of ARGs flowing into natural water.

     https://www.sciencedirect.com/science/article/pii/S0147651320317218