摘要

    堆肥产品在土壤中的应用可能会引起抗生素抗性基因（ARG）向环境的扩散，因此去除堆肥产品中的ARG至关重要。 在本研究中，首次采用臭氧作为去除堆肥产品中ARG的后处理方法。 臭氧处理完成后，臭氧处理可显着去除总的ARG和移动遗传元素（MGE）。 在储存10天后，尽管臭氧处理的堆肥产品中细胞内ARG和MGE的总量增加了，但细胞外ARG和MGE的总量却减少了。 相关分析表明，胞内16S rRNA的减少有助于胞内tetQ和tetW的去除，而臭氧化后其他ARG的变化与MGE的丰度有关。 网络分析表明，臭氧处理后潜在宿主细菌的减少以及NH4 + -N和TOC的减少，有助于细胞内ARGs的去除。

    The soil application of composting product will probably cause the spread of antibiotic resistance genes (ARGs) to environment, thereby it is crucial to remove ARGs in composting product. Ozone was adopted for the first time as a post-treatment method to remove the ARGs in composting product in this study. Ozone treatment significantly removed the total ARGs and mobile genetic elements (MGEs) once ozonation process finished. After 10-day storage stage, although the amount of total intracellular ARGs and MGEs increased, the total extracellular ARGs and MGEs decreased in the ozone-treated compost product. Correlation analysis revealed that the reduction in intracellular 16S rRNA contributed to intracellular tetQ and tetW removal, while the variations of other ARGs after ozonation related to MGEs abundance. Network analysis suggested that the reduction of potential host bacteria, as well as the decline in NH4+-N and TOC after the ozonation, contributed to the intracellular ARGs removal.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420317077