摘要

    在废水处理厂中，大多数病原体和抗生素抗性基因（ARG）转移并集中在废物活性污泥（WAS）中，这将造成严重的公共健康风险。在这项研究中，几种WAS预处理方法能够灭活大肠菌/大肠杆菌。大肠杆菌和ARG，以及随后的大肠菌群/大肠杆菌再生。研究了处理污泥中的大肠杆菌和ARGs / intI1。结果表明，具有连续羟基自由基生成的电子芬顿（EF）可以有效地灭活大肠菌/大肠杆菌。在60分钟内（约4个对数单位）进行大肠杆菌，然后进行甲醇（MT），阳极氧化（AO）和酸化（AT）。动力学分析表明，失活主要发生在开始的10分钟内。但是，所有研究的预处理方法对灭活ARGs / intI1（<2 log单位）的效率均低于大肠菌/ E。大肠杆菌，而EF仍然具有最高的ARGs / intI1降低效率。机械超声治疗（ULS）不能灭活大肠菌/大肠杆菌。 WAS中存在大肠埃希菌，但它可以有效降低ARGs / intI1。大肠菌/大肠杆菌的高再生速率。在10天内，在处理过的WAS中观察到了大肠埃希菌，但在后处理温育过程中ARGs / intI1的丰度持续降低。我们的研究表明，EF可以有效地消毒潜在的病原体，但是，WAS中ARGs / intI1的减少还需要进一步研究。

    In wastewater treatment plants, most of the pathogens and antibiotic resistant genes (ARGs) transferred into and concentrated in waste activated sludge (WAS), which would cause severe public health risks. In this study, the capabilities of several WAS pre-treatment approaches to inactivate coliforms/E. coli and ARGs, as well as the subsequent regrowth of coliforms/E. coli and ARGs/intI1 in treated sludge were investigated. The results showed that electro-Fenton (EF), with continuous hydroxyl radical generation, could efficiently inactivate coliforms/E. coli in 60 min (about 4 log units), followed by methanol (MT), anode oxidization (AO), and acidification (AT). Kinetic analysis showed that the inactivation mainly occurred in the first 10 min. However, the efficiencies of all studied pre-treatment approaches on inactivating ARGs/intI1 (<2 log units) were lower than coliforms/E. coli, whilst EF still had the highest efficiency of ARGs/intI1 reduction. Mechanical ultrasound treatment (ULS) could not inactivate coliforms/E. coli in WAS, but it could efficiently reduce ARGs/intI1. High regrowth rates of coliforms/E. coli were observed in the treated WAS in 10 days, but the abundances of ARGs/intI1 continuously reduced during the after-treatment incubation. Our study showed that EF could efficiently disinfect potential pathogens, however, the reduction of ARGs/intI1 in WAS need further investigation.

    https://www.sciencedirect.com/science/article/abs/pii/S004896972036544X