摘要

    同时包含抗生素抗性细菌（ARB）和抗生素的生物实验室废水是抗生素抗性基因（ARG）的潜在来源。因此，我们确定了从实验室废水中去除5种ARG（sul1，sul2，tetW，tetM，amp）和整合酶编码基因intI1的高压灭菌法（一种常见的消毒方法）的功效。高压灭菌（15分钟，121°C）使包括ARB在内的所有细菌失活，而ARGs甚至在处理60分钟后仍能以有限的减少量持久地存在于废水中。研究了臭氧（O3），超声（US），O3 / US和高压灭菌器，然后是O3降低了实验室废水中ARGs的能力。对于O3和O3 / US，所有研究的ARG的减少率范围为5.44至7.13 log。在目前的实验条件（150 W，53 kHz）下，仅用US进行废水处理并不能降低ARGs。在这四种处理中，高压灭菌和O3处理在最短的时间内显示出最高的还原率。然而，生物实验室废水的深度处理还需要进一步的优化和研究。总的来说，这项研究提供了对ARG来源的新颖见解，并证明了先进的氧化方法对于优化ARG灭活的实验室废水处理很有用。

    Biological laboratory wastewater containing both antibiotic-resistant bacteria (ARB) and antibiotics is a potential source of antibiotic resistance genes (ARGs). Thus, we determined the efficacy of autoclaving, a common disinfection method, in eliminating 5 ARGs (sul1, sul2, tetW, tetM, amp) and the integrase-encoding gene intI1 from laboratory wastewater. Autoclaving (15 min, 121°C) inactivated all bacteria including ARB, whereas ARGs persisted in the wastewater with limited reduction even after 60 min of treatment. Ozonation (O3), ultrasound (US), O3/US, and autoclaving followed by O3 were investigated for their ability to reduce ARGs in laboratory wastewater. With O3 and O3/US, the reduction rate ranged from 5.44 to 7.13 log for all ARGs investigated. Wastewater treatment with US alone did not reduce ARGs under the present experimental conditions (150 W, 53 kHz). Among the four treatments, autoclaving followed by O3 treatment showed the highest reduction rates in the shortest time; however, further optimization and investigation are needed for the advanced treatment of bio-laboratory wastewater. Overall, this study provides novel insights into ARG sources and demonstrates that advanced oxidation methods can be useful to optimize laboratory wastewater treatment for ARG inactivation.

    https://onlinelibrary.wiley.com/doi/abs/10.1002/wer.1451