摘要

    作为内在抗微生物耐药性的重要储存库，土壤受到不断增加的人为活动的影响，这为抗生素抗性基因（ARG）的流行创造了持续的选择压力，从而构成了人类暴露于环境的重要途径。这项研究调查了长江三角洲地区三类ARGs的水平和空间分布与一系列同时发生的化学混合物和土壤特性的关系。在241种农业土壤样品中全部检测出8个ARGs，相对丰度相对于16S rRNA基因标准化为1.01×10-7至2.31×10-1。 sulII和tetG是主要的ARG，平均相对丰度分别为6.67×10-3和5.25×10-3。 ARGs主要存在于长三角地区最具农业和经济活力的地区-太湖和上海市附近的农业土壤中。抗生素，而不是其他同时存在的污染物和土壤特性，仍然是ARGs的主要相关因素，表明它们通过灌溉和施肥或这些来源的抗生素持续选择压力而共同引入土壤，以促进ARGs的增殖。土壤中的ARG。虽然当前的数据集提供了有用的信息以评估ARGs污染的缓解程度，但仍需进行进一步的研究，以揭示有关抗药性从土壤到农产品的潜在转移到人类消费的潜在全貌及其相关健康影响的信息。

    
As an important reservoir of intrinsic antimicrobial resistance, soil is subjected to increasing anthropogenic activities that creates sustained selection pressure for the prevalence of antibiotic resistance genes (ARGs), thus constituting an important environmental dissemination pathway to human exposure. This study investigated the levels and spatial distributions of three classes of ARGs in relation to a range of co-occurring chemical mixtures and soil properties at a regional scale of the Yangtze River Delta (YRD), China. The selected eight ARGs were all detected in 241 agricultural soil samples with relative abundances ranging from 1.01 × 10−7 to 2.31 × 10−1 normalized to the 16S rRNA gene. The sulII and tetG were the dominant ARGs with a mean relative abundance of 6.67 × 10−3 and 5.25 × 10−3, respectively. The ARGs were mainly present in agricultural soils alongside Taihu Lake and Shanghai municipality, the most agriculturally and economically vibrant area of the YRD region. Antibiotics, rather than other co-occurring pollutants and soil properties, remain to be the dominant correlate to the ARGs, suggesting their co-introduction into the soils via irrigation and manure application or the sustained selection pressure of antibiotics from these sources for the proliferation of ARGs in the soils. While the current dataset provided useful information to assess the ARGs pollution for mitigation, future studies are warranted to reveal the complete picture on the potential transfer of antimicrobial resistance from soil to agricultural produces to human consumption and associated health implications

    https://www.sciencedirect.com/science/article/abs/pii/S004896972033521X