摘要

    进行了实验室规模的电化学（EC）消毒实验，以研究其对23种抗生素抗性基因（ARG）的去除效率，这些基因可抵抗8种抗生素，并能影响存活细菌的耐药性。 EC处理以不同的电流密度（D处理）和不同的反应时间（T处理）进行。长时间的电解会导致灭活率高于增加的电流密度，而前者在去除ARG方面效率较低。例如，T20和D80处理的失活率均> 99％，而D80的ARGs相对丰度的降低（从0.54降至4.1）大于T20的ARGs的相对丰度（从5.4降低至5.2）。 EC处理后，对被测抗生素具有抗性的细菌的检出频率降低了9–100％。这主要归因于细菌组成的变化。具有高抗药性频率的细菌的比例减少（如大肠埃希氏菌），而具有低抗药性频率的细菌的比例（如不动杆菌属和假单胞菌）增加。此外，EC消毒后幸存的耐多种抗生素的细菌更少，这也导致了EC实施后废水中抗生素抗性细菌的频率以及耐多菌性指数的显着降低（从0.47到0.35）。治疗（P <0.05）。总的来说，EC消毒不仅降低了ARGs的相对丰度，而且还损害了存活细菌的抗生素耐药性。因此，这可能是控制抗生素耐药性传播的一种有前途的消毒方法。

    Laboratory-scale electrochemical (EC) disinfection experiments were carried out to investigate its removal efficiency for 23 antibiotic resistance genes (ARGs) that confer against 8 classes of antibiotics and its effects on the antibiotic resistance of surviving bacteria. EC treatments were conducted at different current densities (D treatment) and with different reaction times (T treatment). Prolonged electrolysis resulted in a higher inactivation rate than an increased current density, while the former was less efficient in the removal of ARGs. For example, the inactivation ratios for the T20 and D80 treatments were both >99%, while the decrease in the relative abundance of ARGs with D80 (from 0.54 to 4.1) was greater than that with T20 (from 5.4 to 5.2). The detection frequency of bacteria resistant to the tested antibiotics decreased by 9–100% after EC treatment. This was mainly attributed to a change in bacterial composition. The proportion of bacteria with high antibiotic resistance frequencies decreased (such as Escherichia), while that with low resistance frequencies (like Acinetobacter and Pseudomonas) increased. In addition, fewer multi-antibiotic-resistant bacteria survived EC disinfection, which also contributed to the significant decrease in the frequency of antibiotic resistant bacteria as well as in the multi-antibiotic-resistance indices of wastewater samples (from 0.47 to 0.35) after EC treatment (P < 0.05). In total, EC disinfection not only reduced the relative abundance of ARGs, but also impaired the antibiotic resistance of surviving bacteria. So it might be a promising disinfection method for controlling the dissemination of antibiotic resistance.

    https://www.sciencedirect.com/science/article/abs/pii/S138589471933089X