摘要

    抗生素抗性是一个全球性的健康问题，在沉积物生物污染中，抗生素和金属污染在抗生素抗性中的作用是有限的。研究了农村和城市湖泊沉积物的垂直层中抗生素抗性基因（ARGs），抗生素，金属和环境参数之间的关系及其相互关系。一般而言，农村湖泊（太湖= 96％）中的7种抗生素的总浓度显着高于城市湖泊（宣武= 0.3％，乌龙潭= 3％），而金属中的浓度相似（太湖（34） ％），宣武（33％）和五龙潭（33％））。表层沉积物中金属和抗生素的浓度大多高于深层沉积物（对于抗生素；表层= 89％，对于金属而言，深层= 11％；表层= 65％，深层= 35％）。 ARGs在表层和深层沉积物之间无显着差异（表层= 48％，深层= 52％，p <0.05）。 Ni，Cu，Zn，Cr，Mn，As，Cd和Pb污染的潜在生态风险指数表明，太湖和五龙潭具有中等生态风险，而玄武湖则具有较低的生态风险。 Pearson系数和网络分析表明，抗生素，金属，环境参数和ARG之间存在直接和间接的关系，并且这种关系是由关键的环境成分联系在一起的。 tetA，blaTEM，SDZ，TOC，OFL，Cd，OTC，NOR，Ni，sulA，AUR，TC，DOX和TN是影响耐药基因分布的主要因素，形成了复杂的抗生素耐药性网络机制。我们的研究表明，抗生素和重金属广泛分布在表层沉积物中，并且ARGs的增殖受某些关键环境因素的影响。

    Antibiotic resistance is a global health problem, and the role of antibiotics and metal pollution in antibiotic resistance in sediment biocenosis is limited. The occurrence and relationship between antibiotic resistance genes (ARGs), antibiotics, metals and environmental parameters were investigated in vertical layers of sediments in rural and urban lakes. Generally, the total concentrations of seven antibiotics were significantly higher in the rural lake (Lake Taihu = 96%) than in the urban lakes (Xuanwu = 0.3%, Wulongtan = 3%), while similar concentrations were observed for metals (Taihu (34%), Xuanwu (33%) and Wulongtan (33%)). The concentration of metals and antibiotics were mostly higher in the surface sediment layers than the deeper ones (for antibiotics; surface layers = 89%, deeper layer = 11%, for metals; surface = 65%, deep = 35%). The ARGs showed no significant difference between surface and deeper sediments (surface = 48%, deep = 52%, p < 0.05). The potential ecological risk index of Ni, Cu, Zn, Cr, Mn, As, Cd, and Pb contamination showed that Lake Taihu and Wulongtan had moderate ecological risks while Lake Xuanwu had a low ecological risk. Pearson coefficient and network analysis showed that direct and indirect relationship existed among antibiotics, metals, environmental parameters, and ARGs, and the relationship was linked by key environmental components. tetA, blaTEM, SDZ, TOC, OFL, Cd, OTC, NOR, Ni, sulA, AUR, TC, DOX and TN were the major factors that influence the distribution of resistance genes, forming a complex network mechanism of antibiotic resistance. Our study revealed that antibiotics and heavy metals are widely distributed in the surficial sediments and the proliferation of ARGs are influenced by some key environmental components.

    https://www.sciencedirect.com/science/article/pii/S0301479719313015