摘要

    在过去的几十年中，由于潜在的公共卫生风险，不断增加的抗生素抗药性细菌（ARB）逐渐成为全球性威胁。迅速发展的抗生素耐药性及其在环境中的持久性，奠定了开展更多研究以查明可能来源并限制传播的基础。在这种情况下，未被广泛研究的被忽略的遗传物质称为细胞外DNA（eDNA）受到越来越多的关注，因为它可能是通过水平基因转移（HGT）传递到环境细菌的细胞外ARGS（eARG）的重要驱动因素之一。以及环境中各种抗生素抗性基因（ARG）的来源。因此，本综述着重介绍了解决eDNA和编码eARGs在环境中的发生及其对环境电阻系统的影响的研究。在这篇综述中，我们还简要介绍了最近致力于加速eDNA提取的专门技术进步以及减少针对环境抗生素耐药性和潜在生态健康风险的eDNA减少的处理技术的效率。

    Over the past decades, the rising antibiotic resistance bacteria (ARB) are continuing to emerge as a global threat due to potential public health risk. Rapidly evolving antibiotic resistance and its persistence in the environment, have underpinned the need for more studies to identify the possible sources and limit the spread. In this context, not commonly studied and a neglected genetic material called extracellular DNA (eDNA) is gaining increased attention as it can be one of the significant drivers for transmission of extracellular ARGS (eARGs) via horizontal gene transfer (HGT) to competent environmental bacteria and diverse sources of antibiotic-resistance genes (ARGs) in the environment. Consequently, this review highlights the studies that address the environmental occurrence of eDNA and encoding eARGs and its impact on the environmental resistome. In this review, we also brief the recent dedicated technological advancements that are accelerating extraction of eDNA and the efficiency of treatment technologies in reducing eDNA that focuses on environmental antibiotic resistance and potential ecological health risk. 

    https://www.mdpi.com/2076-0817/9/11/874