摘要

    随着北部湾水产养殖的迅速发展，大量抗生素被释放到海洋水生环境中。但是很少有综合研究关注该地区抗生素及其相应ARG的发生及其相关性。我们调查了三种流行的抗生素种类和相应的抗生素抗性基因（ARG）的丰度，分析了北部湾的8条终端河，9条半封闭站点和8条近岸区域中抗生素，ARG和沉积物特性之间的潜在相关性。总抗生素的残留浓度范围为51.04至137.64μg/ kg，TET的浓度高于FQ和SA。传统的ARGs PCR检测显示intI1，sul2，tetB，tetC和tetG在所有样品中的检测频率均为100％。定量分析显示，与tet基因相比，sul基因占主导地位。从河流到公海，抗生素和ARGs的含量都降低了。通过Pearson分析揭示了intI1与ARGs（sul2和tetG）之间的显着相关性（p <.05），这表明intI1基因在ARGs的发生和扩散中发挥了重要作用。通过冗余分析和网络，观察到了ARGs，相应或无关的残留抗生素与沉积物特征之间的显着相关性。由于带有相应ARG的抗生素对健康的潜在危害，控制和治疗措施应引起广泛关注。

    As the rapid development of aquaculture in the Beibu Gulf, large number of antibiotics was released into marine aquatic environment. But little integrated studies concerned on the occurrence and correlation of antibiotics with their corresponding ARGs in this region. We investigated the abundance of three prevalent categories of antibiotics and corresponding antibiotic resistance genes (ARGs), analyzed the potential correlation among antibiotics, ARGs and sediment properties in eight terminal rivers, nine semi-closed sites and eight nearshore areas from Beibu Gulf. Residual concentrations of total antibiotics ranged from 51.04 to 137.64 μg/kg, TETs had higher concentrations than FQs and SAs. Conventional PCR of ARGs revealed intI1, sul2, tetB, tetC and tetG had 100% detection frequency in all samples. Quantitative analysis showed sul genes were dominant compared to tet genes. The abundance of antibiotics and ARGs were decreased from river to open sea. Significant correlations (p < .05) between intI1 and ARGs (sul2 and tetG) were revealed by Pearson analysis, indicating that intI1 gene played a significant role in the occurrence and diffusion of ARGs. Significant correlations among the ARGs, the corresponding or irrelevant residual antibiotics and sediment characteristics were observed by redundancy analysis and network. Due to the potential health risks of antibiotics with corresponding ARGs, the control and treatment measures should deserve wide attention.

    https://www.sciencedirect.com/science/article/abs/pii/S0048969720304095