摘要

    新兴的抗生素抗性细菌（ARB）和抗生素抗性基因（ARG）与微生物污染物一样重要。本文着重介绍了紫外线活化过硫酸盐（UV / PS）（一种先进的氧化工艺），用于从二次废水中去除ARB和ARGs。结果表明，UV / PS对大环内酯类细菌（MRB），耐磺酰胺类细菌（SRB），四环素类细菌（TRB）和喹诺酮类细菌（QRB）的灭活效率分别达到96.6％，94.7％，98.0 10分钟内分别为％和99.9％。 UV / PS还显示出对ARG的显着去除效率。 UV / PS中总ARG的减少达到3.84个数量级，比UV中的减少了0.56 log。特别地，通过UV / PS去除了可能有利于ARG在不同微生物之间水平基因转移的移动遗传元件（MGE）达到了76.09％。高通量测序表明，UV / PS改变了微生物群落。构成人类健康风险的变形杆菌和放线菌的比例分别比紫外线少4.25％和1.6％。共现分析表明ARGs是由细菌类群不同贡献的。在UV / PS系统中，羟基自由基和硫酸根自由基有助于去除细菌和ARG。我们的研究提供了一种新的UV / PS去除废水中ARGs和ARB的方法。

    The emerging antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs) are increasingly appreciated to be as important as microbial contaminants. This paper focused on UV-activated persulfate (UV/PS), an advanced oxidation process, in removing ARB and ARGs from secondary wastewater effluent. Results showed that the inactivation efficiency of macrolides-resistant bacteria (MRB), sulfonamides-resistant bacteria (SRB), tetracyclines-resistant bacteria (TRB) and quinolones-resistant bacteria (QRB) by UV/PS reached 96.6 %, 94.7 %, 98.0 % and 99.9 % in 10 min, respectively. UV/PS also showed significant removal efficiency on ARGs. The reduction of total ARGs reached 3.84 orders of magnitude in UV/PS which is more than that in UV by 0.56 log. Particularly, the removal of mobile genetic elements (MGE) which might favor the horizontal gene transfer of ARGs among different microbial achieved 76.09 % by UV/PS. High-throughput sequencing revealed that UV/PS changed the microbial community. The proportions of Proteobacteria and Actinobacteria that pose human health risks were 4.25 % and 1.6 % less than UV, respectively. Co-occurrence analyzes indicated that ARGs were differentially contributed by bacterial taxa. In UV/PS system, hydroxyl radical and sulfate radical contributed to the removal of bacteria and ARGs. Our study provided a new method of UV/PS to remove ARGs and ARB for wastewater treatment.

     https://www.sciencedirect.com/science/article/abs/pii/S030438942030056X