摘要

    由于潜在的风险，在世界各地的各种基质中经常检测到抗生素抗性基因（ARG），引起了广泛的关注。在关键的生态脆弱地区（例如大陆范围的沿海地区）的水域中，关于ARG污染的信息很少。因此，本研究在冬季和夏季沿中国18000 km海岸线进行了野外采样，以调查目标ARGs在大陆范围内沿海水域的分布。沿海水域中ARGs的绝对丰度表现出剧烈的时空变化，夏季/冬季平均值为8.79×104 / 1.39×105拷贝/ mL，远低于海水养殖区或废水中的尾水。夏季/冬季，沿海水域第1类整合子整合酶基因（intI1）的平均绝对丰度为9.68×103 / 4.15×104拷贝/ mL，仍低于尾水或废水中的平均绝对丰度。喹诺酮抗性基因是沿海水域的主要ARG，在大多数采样点中占总ARG的50％以上。沿海水域的细菌群落在属和属水平上均表现出显着差异。这项研究在沿海水域中的ARGs的丰度与先前报道的其他地区的ARGs相当。尾水和废水可能是沿海水域ARGs的重要来源。这项研究的发现提供了有关大陆范围内沿海水域中ARGs污染状况的全面信息，表明ARGs污染已成为影响沿海地区可持续发展的重要压力。

    Antibiotic resistance genes (ARGs) have been frequently detected in various matrices all over the world to attract wide attention due to the potential risks. Rare information is available on the pollution of ARGs in the waters of critical ecologically fragile regions such as the coastal zone at a continental scale. Therefore, this study performed field sampling during winter and summer along 18000 km coastline of China to investigate the distribution of target ARGs in coastal waters at a continental scale. The absolute abundances of ARGs in coastal waters showed drastic spatio-temporal variation with a mean value of 8.79 × 104/1.39 × 105 copies/mL in summer/winter, much lower than those in tail water from the maricultural zone or wastewater. The average absolute abundance of class 1 integron-integrase gene (intI1) in coastal waters was 9.68 × 103/4.15 × 104 copies/mL in summer/winter, still lower than that in tail water or wastewater. Quinolone resistance genes were the dominant ARGs in coastal waters to account for over 50% of total ARGs in most of sampling sites. Bacterial communities in coastal waters showed significant difference both at phylum and genus levels. Abundances of ARGs in coastal waters of this study were comparable with those in other regions previously reported. Tail water and wastewater might be the essential sources of ARGs in coastal waters. The findings of this study provided comprehensive information on the pollution status of ARGs in coastal waters at a continental scale, indicating that ARGs pollution has become a crucial stress affecting the sustainable development of coastal regions.

    https://www.sciencedirect.com/science/article/abs/pii/S0045653520301004