摘要

    在城市生活垃圾（MSW）堆肥过程中，抗生素抗性基因（ARGs）可能是阻碍MSW堆肥应用的重要因素之一。然而，在工业规模堆肥过程中对ARGs的富集和传播的理解仍不清楚。因此，本研究旨在调查工业规模的城市生活垃圾堆肥厂不同阶段的ARG分布。调查了七个目标ARGs和四个目标移动遗传元件（MGE）和细菌群落。 ARGs和MGEs的丰度在两个有氧嗜热阶段增加，但在堆肥后，大多数ARGs和MGEs的含量降低。网络分析表明ARGs的潜在宿主细菌主要是Firmicutes和放线菌。减少潜在宿主细菌对于去除ARG很重要。 MGE是阻碍ARG去除的重要因素。水溶性S和pH是影响微生物群落变化和ARGs丰度的两个主要理化因素。

    During the municipal solid waste (MSW) composting, antibiotic resistance genes (ARGs) could be one of the concerns to hinder the application of MSW composting. However, the understanding of enrichment and dissemination of ARGs during the industrial-scale composting is still not clear. Hence, this study aimed to investigate the ARG distributions at different stages in an industrial-scale MSW composting plant. Seven target ARGs and four target mobile genetic elements (MGEs) and bacterial communities were investigated. The abundances of ARGs and MGEs increased during two aerobic thermophilic stages, but they decreased in most ARGs and MGEs after composting. Network analysis showed that potential host bacteria of ARGs were mainly Firmicutes and Actinobacteria. The reduction of potential host bacteria was important to remove ARGs. MGEs were an important factor hindering ARG removal. Water-extractable S and pH were two main physicochemical factors in the changes of microbial community and the abundance of ARGs.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852419319522