摘要

    褐煤可用于促进猪粪高固厌氧消化过程中的甲烷生成，但是，褐煤改良剂对HS-AcoD期间ARG命运的影响尚不清楚。在这里，我们探讨了褐煤（0％，8％，16％，32％和64％）对猪粪HS-AcoD期间ARGs命运的影响。结果表明，与0％褐煤处理相比，16％褐煤使ARG的绝对丰度降低了28.71％。变异分区分析表明，微生物群落，流动遗传元素（MGEs）和环境因素的综合作用是形成ARGs模式的主要驱动力。 ARGs的潜在宿主是双歧杆菌，乳杆菌，蒂氏杆菌和链球菌。结构方程模型分析表明，褐煤通过显着降低微生物群落和MGE的含量而间接影响ARG的模式。这些发现提供了对褐煤对HS-AcoD期间ARGs减少的影响的机理理解的见解。

    Lignite could be used to promote methane production during high-solid anaerobic co-digestion (HS-AcoD) of pig manure, however, the effects of lignite amendment on the fate of ARGs during HS-AcoD are unknown. Here, we explored the influence of lignite (0%, 8%, 16%, 32%, and 64%) on the fate of ARGs during HS-AcoD of pig manure. The results showed that 16% lignite reduced the absolute abundance of ARGs by 28.71% compared with the 0% lignite treatment. Variation partitioning analysis suggested the combined effect of microbial community, mobile genetic elements (MGEs) and environmental factors was the major driver shaping the pattern of ARGs. The potential hosts of ARGs were Bifidobacterium, Lactobacillus, Tissierella and Streptococcus. Structural equation models analysis suggested lignite indirectly impacted the pattern of ARGs by significantly reducing the abundance of microbial community and MGEs. These findings give an insight into the mechanistic understanding of the lignite influence on the reduction of ARGs during HS-AcoD.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420301759