摘要

    动物粪便中的抗生素抗性基因（ARG）对人类健康构成巨大威胁。这项研究调查了家禽垃圾堆肥过程中三种水平（5％，10％，15％w / w）添加褐煤对ARGs和细菌群落的影响。褐煤的添加有效地促进了粪便携带的ARG的去除。堆肥65天后，对照（无褐煤）中ARGs的相对丰度降低了8.9％，在5％，10％和15％褐煤处理中分别降低了15.8％，27.7％和41.5％。尽管堆肥后总的流动遗传元素富集，但与对照相比，在10％和15％褐煤处理中，intI-1基因的富集程度显着降低。网络分析表明，放线菌和Firmicutes是ARGs的潜在细菌宿主。冗余分析表明，细菌群落演替在ARGs的转变中起着关键作用。两者合计，这项研究提供证据，褐煤作为添加剂提高了家禽垃圾堆肥过程中ARGs的去除效率。

    Antibiotic resistance genes (ARGs) in animal manure are a great threat to human health. This study investigated the effects of lignite addition at three levels (5%, 10%, 15% w/w) on the profiles of ARGs and the bacterial communities during poultry litter composting. Lignite addition effectively promoted the removal of manure-borne ARGs. After 65 days of composting, the relative abundances of ARGs decreased by 8.9% in control (no lignite), and by 15.8%, 27.7% and 41.5% in 5%, 10% and 15% lignite treatments, respectively. Although the total mobile genetic elements were enriched after composting, the enrichment of the intI-1 gene was significantly lower in the 10% and 15% lignite treatments compared with control. Network analysis indicated that Actinobacteria and Firmicutes were potential bacterial hosts for ARGs. Redundancy analysis showed that bacterial community succession played a key role in the shifts of ARGs. Taken together, this study provides evidence that lignite as additives promoted the removal efficacy of ARGs during composting of poultry litter.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420311135