摘要

    值得揭示暴露于高浓度恩诺沙星（ENR），磺胺嘧啶（SDZ）和三氯生（TCS）的需氧颗粒污泥（AGS）系统中抗生素抗性基因（ARGs）的长期响应。在本研究中，将ppm级的ENR，SDZ和TCS分别添加到三个AGS反应器中。 ENR和SDZ系统中的ARGs呈现先上升然后下降的趋势，这与TCS系统相反。 ENR，SDZ和TCS系统中分别有80％，56％和40％的ARG富集，但是撤出负载后，一些ARG仍保持较高的富集值。 ENR（黄杆菌），SDZ（弯管杆菌和德福球菌）和TCS（德福球菌）系统中的优势细菌可能有助于降低ARGs。 IntI1通过水平基因转移改变了整体ARGs谱。细菌群落和环境因素的相互作用可能是ENR，SDZ和TCS系统中不同ARGs模式的原因。

    It is worth to reveal the long-term responses of antibiotic resistance genes (ARGs) in aerobic granular sludge (AGS) system exposed to high level enrofloxacin (ENR), sulfadiazine (SDZ) and triclosan (TCS). In present study, ppm level ENR, SDZ and TCS were added into three AGS reactors, respectively. ARGs in ENR and SDZ systems showed trends of increasing first and then decreasing, which were contrary to that in TCS system. 80%, 56% and 40% ARGs in ENR, SDZ and TCS systems, respectively, were enriched after loading, but several ARGs still kept high enrichment values after the withdrawn of loadings. The dominant bacteria in ENR (Flavobacterium), SDZ (Candidatus_Competibacter and Defluviicoccus) and TCS (Defluviicoccus) systems might contribute to the reductions of ARGs. IntI1 altered the overall ARGs profiles through horizontal gene transfer. The interactions of bacterial communities and environmental factors might be responsible for the different ARGs patterns in ENR, SDZ and TCS systems.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420308397