摘要

   废水处理厂（WWTP）代表了所有已知类型的抗生素抗性机制，被认为是抗生素抗性基因（ARG）传播的关键点。这项研究的目的是研究在美国路易斯安那州使用氯化法去除两个常规污水处理厂中的1类整合酶基因（intI1）和一组选定的ARG（blaTEM，ermF，mecA和tetA）的方法。我们收集了69个废水样品（进水，次级废水和最终废水各23个），并通过定量聚合酶链反应测定了ARGs的浓度。除mecA以外，所有测试的ARG分别在进水和最终出水样品中检出了83–96％和30–65％。尽管ARGs减少了约3-log10，但两个污水处理厂平均仍释放了废水中研究的总ARGs的3.3±1.7 log10拷贝/ mL。发现氯化对于大幅降低总ARGs至关重要（p <0.05）。相关分析和intI1在治疗过程中持续存在的能力建议使用intI1作为废水中ARGs的标志物，以监测废水中抗生素耐药性的扩散。我们的研究表明，使用氯化法的常规污水处理厂在废水处理过程中不利于抗生素抗性细菌和ARGs的增殖。查看全文

   Wastewater treatment plants (WWTPs) represent all known types of antibiotic resistance mechanisms and are considered as the critical points for the spread of antibiotic resistance genes (ARGs). The purpose of this study is to investigate the removal of a Class 1 integrase gene (intI1) and a selected set of ARGs (blaTEM, ermF, mecA, and tetA) at two conventional WWTPs by using chlorination in Louisiana, USA. We collected 69 wastewater samples (23 each from influent, secondary effluent, and final effluent) and determined the concentrations of ARGs by using quantitative polymerase chain reaction. All tested ARGs, except for mecA, were detected in 83–96% and 30–65% of influent and final effluent samples, respectively. Although the ARGs underwent approximately 3-log10 reduction, two WWTPs on an average still released 3.3 ± 1.7 log10 copies/mL of total ARGs studied in the effluents. Chlorination was found to be critical in the significant reduction of total ARGs (p < 0.05). Correlation analysis and the ability of intI1 to persist through the treatment processes recommend the use of intI1 as a marker of ARGs in effluents to monitor the spread of antibiotic resistance in effluents. Our study suggests that conventional WWTPs using chlorination do not favor the proliferation of antibiotic resistance bacteria and ARGs during wastewater treatment. View Full-Text

    https://www.mdpi.com/2073-4441/12/6/1729