摘要

    抗生素抗性基因（ARGs）的发生和传播是全世界的主要关注点，并且对公共卫生构成了重大威胁。目前的研究集中在中国五粮苏海流域的农业土壤中。在土壤样品中总共鉴定出33种ARG，分别属于8种主要类型，其中以mexF，blaTEM，vanD，sulI，sulII和oprJ为主要。 ARG的丰度范围为2.18×10-6–1.9×10-2拷贝/ 16S rRNA基因拷贝。相关分析的结果证实intI1与ARG（aadA1，aadA2，mphA，sulI和sulII）显着相关，表明这些基因在土壤中的潜在水平转移能力。根据不同采样点与市区的接近程度，我们发现市区附近的细菌群落和ARGs的含量显着增加，这表明农业活动和工业污染可能会改变市区的细菌群落结构和空间分布。 ARGs。此外，ARG与环境因素之间的相关性证实了微生物群落分布与环境因素的关联，例如土壤有机质（SOM），有效磷（AP）和总氮（TN）。 ARGs和细菌类群的共现模式表明，Firmicutes和Proteobacteria的继承可能是在所检查的不同采样点中ARGs变化的潜在驱动力。这些发现有望增进对ARG分布及其与微生物群落演替关系的当前了解。

    The occurrence and dissemination of antibiotic resistance genes (ARGs) constitute a major concern worldwide, and pose crucial threats to public health. The current study focused on this issue in the agricultural soils of Wuliangsuhai watershed in China. A total of 33 ARGs, belonging to 8 major types, were identified in the soil samples, mexF, blaTEM, vanD, sulI, sulII, and oprJ being the predominant ones. The abundance of ARGs ranged from 2.18 × 10−6–1.9 × 10-2 copies/16S rRNA gene copies. Results of correlation analyses confirmed that intI1 significantly correlated with ARGs (aadA1, aadA2, mphA, sulI, and sulII), indicating the potential horizontal transfer capability of these genes in soil. Based on the proximity of different sampling sites with urban areas, we found the abundance of bacterial community and ARGs to significantly increase in the sites proximal to urban area, which indicated the agricultural activities and industrial pollution might alter the bacterial community structure and spatial distribution of ARGs. In addition, the correlation between ARGs and environmental factors confirmed the association of microbial community distribution with environmental factors, such as soil organic matter (SOM), available phosphorus (AP), and total nitrogen (TN). The co-occurrence pattern of ARGs and bacterial taxa indicated the succession of Firmicutes and Proteobacteria to possibly be the potential driver for variation in ARGs in the different sampling sites examined. These findings are expected to improve the current understanding of ARG distribution and their relationship with microbial community succession.

    https://www.sciencedirect.com/science/article/abs/pii/S0167880920300694