摘要

    在这项研究中，对废水和污泥样品中的抗生素抗性基因（ARG）进行了研究，以探讨头孢氨苄（CFX）对膨胀颗粒污泥床（EGSB）反应器中处理抗生素废水的ARG扩散和去除的影响。结果表明，废水中添加CFX影响了β-内酰胺类ARG和其他类型ARG的去除量。此外，废水中添加CFX对污泥中目标ARG的总浓度没有明显影响，但与某些典型ARG的积累有关。根据基因盒阵列文库分析，通过在废水中添加CFX，增加了intI1基因携带的基因盒的多样性。此外，还研究了ARG和细菌属之间的共现模式。结果表明，废水中的CFX不仅影响ARGs潜在宿主菌的数量，而且改变了ARGs潜在宿主菌的类型。进水，出水和污泥中ARG的相关性分析表明，对于blaCTX-M，sul2，qnrS和AmpC基因，通过降低污泥中EGSB反应器的浓度，可以提高它们在EGSB反应器处理抗生素废水系统中的去除量。

    In the study, antibiotic resistance genes (ARGs) were examined in wastewater and sludge samples to explore the effect of cephalexin (CFX) on the spreading and removal of ARGs in the Expanded Granular Sludge Bed (EGSB) reactor treating antibiotics wastewater. The result showed that the addition of CFX in the wastewater affected the removal amount of β-lactam ARGs and other types ARGs. Besides, the addition of CFX in the wastewater had no obviously effect on total concentration of targeted ARGs in the sludge, but it was related to the accumulation of some typical ARGs. Based on gene cassette array libraries analysis, the diversity of gene cassettes carried by intI1 gene was increased by the addition of CFX in the wastewater. Furthermore, the co-occurrence patterns between ARGs and bacterial genus were also investigated. The results showed the CFX in the wastewater not only affected the number of potential host bacteria of ARGs, but also changed the types of potential host bacteria of ARGs. The correlation analysis of ARG in influent, effluent and sludge showed that, for blaCTX-M, sul2, qnrS and AmpC genes, their removal amount in EGSB reactor treating antibiotic wastewater system might be enhanced by reducing their concentration in the sludge.

    https://www.sciencedirect.com/science/article/abs/pii/S0147651320305789