摘要

    为了减少过量污泥中的抗生素抗性基因（ARGs）和1类整合素（intI1）的风险，本研究探讨了碱性，微波，芬顿和综合处理对ARGs去除的影响。同时，确定了由不同处理引起的微生物群落组成的改变，以解释ARG的潜在宿主。相比之下，碱和微波处理增加了几种ARGs和intI1的丰度，但芬顿处理大大降低了ARGs和intI1的丰度。此外，发现碱性整合的芬顿和微波整合的芬顿处理强烈地降低了靶基因的丰度，并且降低的量仅高于芬顿。另外，通过碱和微波处理，微生物群落结构略有变化，但通过芬顿处理显着改变。经过芬顿处理后，纤毛虫和放线菌的相对丰度明显升高，表明纤毛虫和放线菌可能是其余ARGs的宿主。可以预期，这项研究将为降低环境中过量污泥进入ARGs的风险提供重要信息。

    To reduce the risk of antibiotic resistance genes (ARGs) and class 1 integrons (intI1) in excess sludge, this study explored the effect of alkaline, microwave, fenton and the integrated treatments on ARGs removal. At the same time, the alteration of microbial community composition caused by different treatments was determined to explain the potential hosts of ARGs. By comparison, alkaline and microwave treatment increased the abundance of several ARGs and intI1, but fenton treatment largely reduced ARGs and intI1 abundance. Moreover, it was found that alkaline integrated fenton and microwave integrated fenton treatment strongly reduced target genes abundance, and the reduced amount was higher than fenton only. Additionally, the microbial community structure was slightly changed by alkaline and microwave treatment, but was remarkably altered by fenton treatment. After fenton treatment, the relative abundance of Firmicutes and Actinobacteria was obviously elevated, suggesting that Firmicutes and Actinobacteria might be the possible hosts for the remaining ARGs. It is expected that this study could provide crucial information for reducing the risk of ARGs from excess sludge entering in the environment.

    https://www.sciencedirect.com/science/article/abs/pii/S0964830519316671