摘要

    在这项研究中，研究了污水污泥嗜热厌氧消化（AD）启动过程中不同温度变化对抗生素抗性基因（ARGs）和细菌群落动态的影响。尽管两个嗜热反应器在间歇模式下显示出不同的ARGs去除效率，但在连续搅拌釜反应器（CSTR）模式下，ARGs的去除效率和还原模式均相似，从而导致ARGs相对丰度总和的显着降低。通过网络分析探索细菌群落与某些特定ARGs之间的相关性，发现细菌群落的组成在抗生素抗药性相对丰度的波动中起着至关重要的作用，表明垂直基因转移可促进ARGs的形成。 。为了促进消除ARG，在嗜热性AD中至关重要的是，将即使在长期操作下仍能持续存在的宿主减至最少。

    In this study, the effects of different temperature transitions on the dynamics of antibiotic resistance genes (ARGs) and bacterial community were investigated during start-up of thermophilic anaerobic digestion (AD) of sewage sludge. Although two thermophilic reactors showed dissimilar removal efficiencies of ARGs in batch mode, both the removal efficiency and reduction patterns of ARGs were similar in continuous stirred tank reactor (CSTR) mode, resulting in significant reduction of the total sum of the relative abundance of ARGs. Using network analysis to explore the correlation between bacterial community and some specific ARGs revealed that composition of the bacterial community played a vital role in the fluctuations in the relative abundance of the antibiotic resistome, demonstrating that shaping the development of ARGs was facilitated by vertical gene transfer. To facilitate eliminating ARGs, minimizing their hosts which persist even under long-term operations is vital in thermophilic AD.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420307823