摘要

    堆肥不能完全有效地减少动物粪便中的抗生素抗性基因（ARG）。这项工作研究了不同处理条件对以花椰菜和玉米秸秆为填充剂堆肥猪粪时ARGs命运的影响。结果表明，与对照相比，添加微生物制剂或玉米秸秆与花椰菜的比例（1:12）可以显着降低大多数ARG的绝对丰度（平均480倍）。主成分分析表明，细菌群落与ARG的丰度显着相关，这表明微生物群落对协同堆肥期间ARG的变化有影响。冗余和网络分析证实了单个ARGs（qnrS，blaAmpC，blaTEM-1）的变化模式受到宿主细菌（假单胞菌，克雷伯菌和Halocella）的选择性和环境变量（TN，NH3-N，TOC和pH）的影响）。这些发现有助于优化堆肥条件，从而降低了ARGs扩散的风险。

    Composting is not completely effective in reducing antibiotic resistance genes (ARGs) in animal manure. This work studied the effects of different treatment conditions on the fate of ARGs in composting swine manure with cauliflower and corn straw as bulking agents. The results showed that the addition of microbial agents or the ratio of corn stalks to cauliflower (1:12) could significantly decrease the absolute abundances of most ARGs (an average of 480 times) compared with the control treatment. Principal component analysis indicated that bacterial communities were significantly correlated with ARG abundance, suggesting that microbial communities have an impact on ARG variation during co-composting. Redundancy and Network analysis confirmed the changing patterns of individual ARGs (qnrS, blaAmpC, blaTEM-1) were influenced by the selectivity of host bacteria (Pseudomonas, Klebsiella, and Halocella) and environmental variables (TN, NH3-N, TOC, and pH). These findings helped to optimize composting conditions, thereby reducing the risk of ARGs spread.

    https://www.sciencedirect.com/science/article/abs/pii/S096085241931898X