摘要

    背景：水生生态系统被认为是最重要的抗生素耐药性库之一
基因（ARG）。饮用水源可以作为ARGs从环境向人类转移的关键点。在
本研究调查了长江三角洲青草沙水库三个季节中抗生素和ARGs的分布，以表征其污染模式。
结果：磺胺甲恶唑，磺胺单甲恶碱和青霉素G钾盐是该菌的主要抗生素。
这个水库。磺酰胺抗性基因（sul1和sul2）是最普遍和最主要的基因。更高
与其他站点相比，最靠近infow的站点检测到了ARG的总相对丰度。全面的，
5月的抗生素浓度相对低于11月和2月。相关性
分析表明，sul1，ermB和mphA与相应的抗生素在2月和sul1中呈正相关，
sul2，ermB和mphA也与intI1正相关。
结论：总之，青草沙水库中抗生素和ARGs普遍存在。我们的结果表明
饮用水水库可能是抗药性的基因库，而流动基因元件intI1可以
作为促进各种ARG广泛传播的媒介。由于发现ARGs的丰度更高
在水库的信息流中，与出水口相比，认为水库可以作为功能区
有助于消除或稀释ARG。
关键词：抗生素抗性基因饮用水水库抗生素残留

    Background: Aquatic ecosystems are considered to be among the most important reservoirs of antibiotic resistance
genes (ARGs). Drinking water sources could serve as critical points of ARGs transfer from environment to human. In
this study, the distribution of antibiotics and ARGs across Qingcaosha Reservoir located in the Yangtze River Delta during three seasons was investigated to characterize the pollution patterns.
Results: Sulfamethoxazole, sulfamonomethoxine and penicillin G potassium salt were the dominant antibiotics in
this reservoir. Sulfonamide resistance genes (sul1 and sul2) were the most prevalent and predominant genes. Higher
total relative abundance of the ARGs was detected in the site closest to the infow than those in other sites. Overall,
the concentrations of antibiotics in May were relatively lower than those in November and February. Correlation
analysis indicated sul1, ermB and mphA had positive correlation with corresponding antibiotics in February and sul1,
sul2, ermB and mphA was also positively correlated to intI1.
Conclusion: In conclusion, antibiotics and ARGs were widespread in Qingcaosha Reservoir. Our results indicated that
the drinking water reservoir might serve as gene reservoir for antibiotic resistance and mobile gene element intI1 can
serve as a medium to contribute to the widespread of various ARGs. As the ARGs abundance was found to be higher
in the infow of the reservoir compared to the outfow, it is considered that reservoir could serve as a functional area
contributing to the elimination or dilution of ARGs.
Keywords: Antibiotic resistance genes, Drinking water reservoir, Antibiotic residues

    https://link.springer.com/content/pdf/10.1186/s12302-020-00357-y.pdf