摘要

    微塑料（MPs）和抗生素抗性基因（ARGs）都被认为是日益引起人们关注的新兴污染物，因为它们的综合污染对生态环境和人类健康构成了严重的风险。在这项研究中，高通量定量PCR技术用于研究河水中ARGs的多样性和丰度，并在其中添加了两种不同的微塑料（PVC和PVA）进行曝气培养。结果表明，河水中的ARGs种类繁多，微塑料可以诱导更多类型的ARGs。尽管在通气条件下培养了14 d的所有三种处理中，ARGs的数量和丰度都下降了，但与未处理样品相比，MP曝气的处理中ARGs的总丰度要高于未MPs曝气的处理。在使用水溶性微塑料（PVA）处理的样品中。观察到ARGs的丰富与流动遗传元件（MGEs）之间的显着相关性，这意味着MGEs的发生可能通过河水中的水平基因转移（HGT）潜在地影响ARGs的传播和分布

    Microplastics (MPs) and antibiotic resistance genes (ARGs) are both considered emerging contaminants of increasing concern because their combined pollution poses a serious risk to the ecological environment and human health. In this study, high-throughput quantitative PCR techniques were used to investigate the diversity and abundance of ARGs in river water, to which two different microplastics (PVC and PVA) were added for aerated incubation. The results showed that ARGs in river water were diverse, and microplastics could induce more types of ARGs. Although the number and abundance of ARGs decreased in all three treatments, which were cultivated for 14 d by aeration, compared to those in non-treated samples, the total abundance of ARGs in treatments aerated with MPs were higher than those aerated without MPs, especially in the samples treated with water-soluble microplastics (PVA). Significant correlations between the abundance of ARGs and mobile genetic elements (MGEs) were observed, implying that the occurrence of MGEs may potentially affect the transmission and distribution of ARGs through horizontal gene transfer (HGT) in river water.

    https://europepmc.org/article/med/33124288