摘要

    微生物电解池（MEC）系统是一种新兴的污染控制技术。 但是，关于处理污水污泥的MEC中抗生素抗性基因（ARG）变异的信息仍然非常有限。 在这项研究中，研究了在不同施加电压（0–1.5 V）下，MEC中ARG的命运及其与微生物的相关性。 有效去除了大多数目标ARG，但是tetB，tetM和tetQ在悬浮污泥中的富集量高达2.05 log单位。 除富含悬浮污泥的tetQ和tetM外，大多数ARGs主要分布在电极上。 污水污泥中残留抗生素的选择压力可以忽略不计。 水平基因转移已验证在MEC中sul1，sul2，tetA和tetC的扩散。 网络分析表明，ARGs的潜在宿主主要是拟杆菌，硬毛菌和变形杆菌。 最近发现一些与电子转移有关的属是MEC中潜在的ARGs宿主。

    Microbial electrolysis cells (MECs) system is an emerging pollution control technology. However, information on the variation of antibiotic resistance genes (ARGs) in MECs treating sewage sludge is still very limited. In this study, the fate of ARGs and their correlation with microbes in MECs under different applied voltages (0–1.5 V) were studied. Most target ARGs were effectively removed, but tetB, tetM and tetQ were enriched up to 2.05 log units in suspended sludge. Most ARGs were mainly distributed on electrodes, except tetQ and tetM enriched in suspended sludge. The selective pressure of residual antibiotics in the sewage sludge was negligible. Horizontal gene transfer was validated for the spread of sul1, sul2, tetA and tetC in MECs. Network analysis revealed that the potential hosts of ARGs mainly belonged to Bacteroidetes, Firmicutes and Proteobacteria. Some genera related to electron transfer were newly found to be the potential ARGs hosts in MECs.

    https://www.sciencedirect.com/science/article/abs/pii/S096085242031110X