摘要

    这项研究的重点是在食品废水（FWW）的全面AD中ARGs的命运。残留物是从两种不同的处理FWW的全尺寸嗜热性AD中收集的。使用定量聚合酶扩增了十个选定的ARG，包括四环素抗性基因（tetM，tetX，tetQ，tetH和tetG），磺酰胺类抗性基因（sul1和sul2），喹诺酮类抗性基因（qnrD）和大环内酯类抗性基因（ermB和ermC）。连锁反应（qPCR）。此外，选择了1类整合子整合酶基因（intI1）作为代表性的移动基因元件。在两阶段（产酸-产甲烷）AD中观察到ARGs和intI1显着降低，尤其是未检测到tetG，tetH，tetM，tetQ，tetX和intI1。此外，ARG与intI1之间的显着正相关（p <0.01）表明水平基因转移（HGT）的可能性很大。此外，逐步多元线性回归分析揭示了与AD期间个体ARG和intI1的命运有关的重要因素。

    This study focus on the fate of ARGs in the full-scale AD of food wastewater (FWW). Residue was collected from two different full-scale thermophilic AD treating FWW. Ten selected ARGs, including tetracycline resistance genes (tetM, tetX, tetQ, tetH and tetG), sulfonamide resistance genes (sul1 and sul2), quinolone resistance genes (qnrD) and macrolide resistance genes (ermB and ermC), were amplified using quantitative polymerase chain reaction (qPCR). Furthermore, the class 1 integron-integrase gene (intI1) was selected as a representative mobile gene element. Remarkable reduction in the ARGs and intI1 was observed in two-stage (acidogenic–methanogenic) AD, particularly, tetG, tetH, tetM, tetQ, tetX and intI1 not detected. Additionally, significant positive correlation (p < 0.01) between ARGs and intI1 suggested a strong likelihood of horizontal gene transfer (HGT). Furthermore, stepwise multiple linear regression analysis revealed significant factors related to the fate of individual ARGs and intI1 during AD.

    https://www.sciencedirect.com/science/article/abs/pii/S096085242030849X