摘要

    使用宏基因组学方法评估了嗜温厌氧消化（AD）对来自两个不同奶牛场的粪便抵抗力的影响。 在未处理（未消化）的粪肥中共检测到针对17种不同类别抗生素的187个独特的抗生素抗性基因（ARG）。 结果表明，不管乳牛粪的来源如何，嗜温性AD都能减少或富集某些ARG的相对丰度。 这些变化的主要驱动力与AD过程中微生物群落的演变密切相关。 通过分析微生物群和ARG的共存来建议推定的ARG宿主。 最后，网络分析表明，嗜温性AD还可以减少可能位于同一遗传元件中的不同类ARG的同时出现。 我们的结果提供了有价值的见解，在嗜温性AD期间驱动ARGs的多样性和丰富性的微生物机制。

     The effect of mesophilic anaerobic digestion (AD) on the resistome profile of manures from two different dairy farms was evaluated using a metagenomic approach. A total of 187 unique Antibiotic resistance genes (ARGs) for 17 different classes of antibiotics were detected in raw (undigested) manures. The results indicate that regardless of the origin of the dairy manure, mesophilic AD was capable of reducing or enriching the relative abundance of some ARGs. The main driver of these changes was strongly correlated with the evolution of the microbial community during the AD process. Putative ARG hosts were suggested by analyses of the co-occurrence of microbial groups and ARGs. Finally, network analyses revealed that mesophilic AD could also reduce the co-occurrence of different groups of ARGs potentially located in the same genetic elements. Our results provide valuable insights into the microbial mechanisms driving the diversity and abundance of ARGs during mesophilic AD.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420311615