摘要

    废水中抗生素抗性细菌（ARB）的增殖和抗生素抗性基因（ARGs）的流行是公认的因素，这些因素导致全球医疗保健所用抗生素的效力降低。为了设计缓解措施以对抗其传播，需要了解与ARB和ARG扩散相关的人类健康风险。使用基因组DNA的PCR分析，从尼日利亚的制药废水中获得的60种细菌分离物中，评估了对常用的六类抗生素有活性的41种ARG的患病率。在研究的每种抗生素类别中，最常从细菌分离物中检测到的ARGs包括catA1（58.3％）；苏（31.7％）; tet（E）（30％）； aac（3）-IV（28.3％）； ermC（20％）； blaTEM，blaCTX-M和blaNDM-1，各占18.3％；它们分别编码对氯霉素，磺酰胺，四环素，氨基糖苷，大环内酯-林可酰胺-链霉菌素和β-内酰胺和青霉素的抗性。不动杆菌属，登录号MH396735表达了所有细菌分离物中最高的ARG数，具有至少一个编码该研究中所有类型抗生素抗性的基因。这项研究强调了ARB和ARGs在废水中测试的抗生素中的广泛分布，使ARGs的制药废水库有可能从共生微生物转移到人类病原体

    The proliferation of antibiotic-resistant bacteria (ARB) and the prevalence of antibiotic resistance genes (ARGs) in wastewaters are well-established factors that contribute to the reduced potency of antibiotics used in healthcare worldwide. The human health risk associated with the proliferation of ARB and ARGs need to be understood in order to design mitigation measures to combat their dissemination. Using the PCR analysis of genomic DNA, the prevalence of 41 ARGs active against the commonly used six classes of antibiotics was evaluated in 60 bacterial isolates obtained from pharmaceutical wastewaters in Nigeria. The ARGs most frequently detected from the bacterial isolates in each of the antibiotic classes under study include catA1 (58.3%); sulI (31.7%); tet(E) (30%); aac(3)-IV (28.3%); ermC (20%); blaTEM, blaCTX-M, blaNDM-1 at 18.3% each; which encode for resistance to chloramphenicol, sulfonamides, tetracycline, aminoglycoside, macrolide-lincosamide-streptogramin and β-lactams and penicillins, respectively. Acinetobacter spp., accession number MH396735 expressed the highest number of ARGs of all the bacterial isolates, having at least one gene that encodes for resistance to all the classes of antibiotics in the study. This study highlights wide distribution of ARB and ARGs to the antibiotics tested in the wastewater, making pharmaceutical wastewater reservoirs of ARGs which could potentially be transferred from commensal microorganisms to human pathogens

    https://www.sciencedirect.com/science/article/abs/pii/S0960852420311615