摘要

    构建了具有高处理效率的三维生物膜电极反应器（3D-BERs），用于处理含有磺胺嘧啶（SDZ）和环丙沙星（CIP）与锌（Zn）共同暴露的废水。结果表明，与目标抗生素和Zn的共同暴露增加了目标抗生素抗性基因（ARG）的绝对和相对丰度。另外，与普通厌氧反应器相比，3D-BER阴极的目标ARG丰度更高，而出水中总ARG的丰度降低。同时，冗余分析结果表明，带有锌和抗生素的积累极大地影响了带有ARGs的细菌的组成，而锌和抗生素的积累主导了ARGs的变化。另外，通过网络分析发现带有宿主细菌的ARGs在从废水中去除后会部分沉积在电极基材上。因此，3D-BER具有同时消除抗生素和锌的能力，并大大降低了ARGs扩散的风险。

    Three-dimensional biofilm electrode reactors (3D-BERs) with high treatment efficiency were constructed to treat wastewater containing sulfadiazine (SDZ) and ciprofloxacin (CIP) coexposure with Zinc (Zn). The results showed that coexposure to target antibiotics and Zn increased the absolute and relative abundances of target antibiotic resistance genes (ARGs). Additionally, the target ARG abundances were higher on cathode of 3D-BER compared with ordinary anaerobic reactor while the abundances of total ARGs were decreased in the effluent. Meanwhile, redundancy analysis results revealed that the composition of bacteria carrying ARGs was greatly influenced in the cathode by the accumulation of Zn and antibiotic, which dominated the changes of ARG abundances. Additionally, ARGs with their host bacteria revealed by network analysis were partially deposited on electrode substrates when being removed from wastewater. Thus, 3D-BER exhibits capability of simultaneously eliminating antibiotic and Zn, and greatly reduces the risks of ARGs spread.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852419315202