摘要

    这项研究调查了猪粪嗜热性AD期间甲烷生成量和ARGs的减少情况，方法是添加不同的铜盐（硫酸铜，甘氨酸铜和这两种盐的1：1混合物）。结果表明，通过添加混合铜盐，甲烷的产量增加了28.78％。混合铜盐组有效降低了总ARGs含量26.94％，表明混合铜盐并没有提高潜在的ARGs风险。混合铜盐对AD性能和ARGs去除的积极影响可能归因于生物利用度低。微生物群落分析表明，混合铜群中梭菌_MBA03和甲烷菌的最高丰度可能导致甲烷产量增加。 Spearman的等级相关分析阐明了由环境因素引起的微生物群落演替是形成ARGs轮廓的主要驱动力。因此，混合铜盐可以替代动物饲料添加剂中的无机铜盐。

    This study investigated methane production and ARGs reduction during thermophilic AD of swine manure with the addition of different Cu salts (cupric sulfate, cupric glycinate, and the 1:1 mixture of these two salts). Results showed methane production was increased by 28.78% through adding mixed Cu salts. The mixed Cu group effectively reduced total ARGs abundance by 26.94%, suggesting mixed Cu salts did not promote the potential ARGs risk. The positive effects of mixed Cu salts on AD performance and ARGs removal might be ascribed to the low bioavailability. Microbial community analysis indicated the highest abundances of Clostridia_MBA03 and Methanobacterium in the mixed Cu group might cause the increased methane production. Spearman’s rank correlation analysis elucidated the succession in microbial community induced by environmental factors was the main driver for shaping ARGs profiles. Thus, mixed Cu salts could be an alternative to replace the inorganic Cu salt in animal feed additives.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852419319571