摘要

    诸如抗生素抗性细菌（ARB）和抗生素抗性基因（ARGs）等新兴污染物正在成为全球性的环境问题。在这项研究中，辉光放电血浆（GDP）用于在0.9％的无菌盐水中降解具有抗性基因（tetA，tetR，aphA）和转座酶基因（tnpA）的抗生素抗性大肠杆菌（E. coli）。结果表明，GDP能够灭活抗药性大肠杆菌并去除ARGs，并降低了基因转移的风险。 15S放电处理后，由16S rRNA确定的大肠杆菌水平下降了约4.7 log。单叠氮化丙锭-定量聚合酶链反应（PMA-qPCR）测试表明，在15分钟内破坏了4.8个多对数大肠杆菌的细胞结构。随着30分钟放电处理，tetA，tetR，aphA，tnpA基因的减少分别增加到5.8、5.4、5.3和5.5log。还研究了从高盐度废水中去除ARGs的方法。在30分钟内，ARG的总丰度降低了3.9 log。清除测试表明，羟基自由基（·OH）是最可能导致细菌失活和ARGs降解的物质。另外，在放电过程中形成的活性氯（Cl·和Cl2）也可能导致失活和降解。

    Emerging contaminants such as antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs) are becoming a global environmental problem. In this study, the glow discharge plasma (GDP) was applied for degrading antibiotic resistant Escherichia coli (E. coli) with resistance genes (tetA, tetR, aphA) and transposase gene (tnpA) in 0.9% sterile saline. The results showed that GDP was able to inactivate the antibiotic resistant E. coli and remove the ARGs and reduce the risk of gene transfer. The levels of E. coli determined by 16S rRNA decreased by approximately 4.7 logs with 15 min of discharge treatment. Propidium monoazide - quantitative polymerase chain reaction (PMA-qPCR) tests demonstrated that the cellular structure of 4.8 more logs E. coli was destroyed in 15 min. The reduction of tetA, tetR, aphA, tnpA genes was increased to 5.8, 5.4, 5.3 and 5.5 logs with 30 min discharge treatment, respectively. The removal of ARGs from high salinity wastewater was also investigated. The total abundance of ARGs was reduced by 3.9 logs in 30 min. Scavenging tests indicated that hydroxyl radicals (·OH) was the most probable agents for bacteria inactivation and ARGs degradation. In addition, the active chlorine (Cl· and Cl2) which formed during the discharge may also contribute to the inactivation and degradation.

    https://www.sciencedirect.com/science/article/abs/pii/S004565352030669X