摘要

    这项研究采用了宏基因组学的方法来研究worm在脱水污泥的堆肥过程中对ARGs和HPB的影响。结果表明，在污泥ver堆中发现了139种ARGs，隶属于30个类别。与对照组相比，污泥ver堆中ARGs的总含量下降了41.5％。此外，通过ver堆邮也减少了质粒和整合素的类型和序列。蛋白质细菌和放线菌是污泥ver堆中最主要的ARG宿主。此外，earth降低了总HPB的丰度并改变了它们的多样性，从而导致污泥ver堆中肠杆菌科细菌的丰度更高。然而，污泥ver堆仍是ARG和HPB富集的，这表明仍存在用于农业目的的环境风险。 worm活动引起的观察到的微生物群落变化和活动性遗传元素的减少是减轻ARG污染的主要原因。

    This study used a metagenomic approach to investigate the effects of earthworms on ARGs and HPB during the vermicomposting of dewatered sludge. Results showed that 139 types of ARGs were found in sludge vermicompost, affiliated to 30 classes. Compared with the control, the total abundance of ARGs in sludge vermicompost decreased by 41.5%. Moreover, the types and sequences of plasmids and integrons were also decreased by vermicomposting. Proteobacteria and Actinobacteria were the most dominant hosts of ARGs in sludge vermicompost. In addition, earthworms reduced the total HPB abundance and modified their diversity, thus leading to higher abundance of Enterobacteriaceae in sludge vermicompost. However, the sludge vermicompost was still ARG and HPB enriched, indicating a remaining environmental risk for agricultural purpose. The observed change of microbial community and the reduction of mobile genetic elements caused by earthworm activity are the main reasons for the alleviation of ARG pollution during vermicomposting.

    https://www.sciencedirect.com/science/article/abs/pii/S0960852419316815