摘要

    抗生素抗性基因（ARG）的广泛出现已成为重要的临床问题。在原始土壤环境中研究ARGs可以有助于更好地了解土壤固有的电阻组。在这项研究中，从高海拔和相对原始的藏族地区收集了10个土壤样品，并进行了宏基因组测序和生物信息学分析，以调查微生物的多样性，ARG的丰度和多样性以及ARG的移动潜力，如不同的移动指示。遗传元件（MGEs）。在藏族土壤样品中共检测到48种ARG类型，相对数量为ARG 0.05 / 0.28拷贝/ 16S rRNA基因拷贝。观察到的ARGs主要与包括糖肽和利福霉素在内的抗生素有关。最丰富的ARG是vanRO和vanSO。 MGEs和可能与质粒相关的ARG含量低表明原始土壤中ARGs的水平基因转移风险低。 Pearson相关性和冗余度分析表明，温度和总有机碳是控制微生物多样性和ARG丰度和多样性的主要环境因素。

    Widespread occurrence of antibiotic resistance genes (ARGs) has become an important clinical issue. Studying ARGs in pristine soil environments can help to better understand the intrinsic soil resistome. In this study, 10 soil samples were collected from a high elevation and relatively pristine Tibetan area, and metagenomic sequencing and bioinformatic analyses were conducted to investigate the microbial diversity, the abundance and diversity of ARGs and the mobility potential of ARGs as indicated by different mobile genetic elements (MGEs). A total of 48 ARG types with a relative abundance of 0.05–0.28 copies of ARG/copy of 16S rRNA genes were detected in Tibetan soil samples. The observed ARGs were mainly associated with antibiotics that included glycopeptide and rifamycin; the most abundant ARGs were vanRO and vanSO. Low abundance of MGEs and potentially plasmid-related ARGs indicated a low horizontal gene transfer risk of ARGs in the pristine soil. Pearson correlation and redundancy analyses showed that temperature and total organic carbon were the major environmental factors controlling both microbial diversity and ARG abundance and diversity.

    https://academic.oup.com/femsec/article-abstract/96/10/fiaa172/5894923