摘要

       采用单级厌氧消化、一级厌氧消化和二级厌氧消化以及微波- H2O2预处理3种工艺，通过qPCR和高通量测序方法，研究了重金属、int1和微生物群落共同选择的抗生素抗性基因( ARGs )分布特征及其驱动因素。结果表明，MW- H2O2能降低所有ARGs的绝对基因拷贝数，同时增加大多数ARGs的相对丰度。经过AD处理后，ARGs 总含量和相对丰度均有所提高，而两阶段AD处理显示优于ARGs丰度的优势。此外，AD比MW - H2O2对潜在致病菌的抑制作用更强。AD可降低int1对ARGs扩散的作用，mantel试验和procrustes分析表明，ARGs丰度的变化与细菌群落的差异密切相关。

       Three anaerobic digestion (AD) processes of waste activated sludge (WAS) were established including the control (mono-WAS), one-stage AD and two-stage AD along with microwave-H2O2 pre-treatment (MW-H2O2) to investigate the profiles and drivers of antibiotic resistance genes (ARGs) distribution concerning co-selection from heavy metals,intI1 and microbial community through qPCR and high-throughput sequencing method. Results showed that MW-H2O2 could reduce the absolute gene copies of all ARGs while increased the relative abundance of most ARGs. After subsequent AD, both total ARGs quantities and relative abundance were enriched while two-stage AD showed some advantages over ARGs abundance reduction. Besides, AD was more effective on the potential pathogens reduction than MW-H2O2. AD could reduce the role of intI1 on the spread of ARGs, while mantel test and procrustes analysis indicated that the variation of ARGs abundance was closely associated with the discrepancy of bacterial community.
 
https://www.sciencedirect.com/science/article/pii/S0960852417308258