摘要

  本研究探讨了常用抗生素（林可霉素、氯四环素、磺胺甲恶唑和环丙沙星）及其对堆肥过程中抗生素耐药性的集体影响。在前7天，环丙沙星在研究的抗生素中对理化因素的影响最大；多重抗生素处理中抗生素抗性基因（ARGs）的去除明显少于单一抗生素处理；尤其是，单次环丙沙星治疗对核糖体保护基因（tetW 和 tetO）的最大去除发生。在堆肥结束时，不同处理中ARGs的去除率相似（29.71-99.79%）（p大于0.05）；根据网络分析，Chloroflexi 成为主要门，它与 ARGs 去除密切相关。 ARGs的潜在宿主菌因抗生素不同而异；特别是，多种抗生素的存在增加了 ermA、sul1 和 tetO 的潜在宿主细菌。最重要的是，不同抗生素的集体效应导致堆肥中抗生素抗性的增强。

   This study explored commonly-used antibiotics (lincomycin, chlorotetracycline, sulfamethoxazole, and ciprofloxacin) and their collective effects on antibiotic resistance during composting. In the first 7 days, ciprofloxacin showed the greatest influence on the physicochemical factors among the studied antibiotics; the removal of antibiotic resistance genes (ARGs) in the multiple-antibiotic treatment was significantly less than single-antibiotic treatments; especially, the largest removal of ribosomal protection genes (tetW and tetO) occurred in single ciprofloxacin treatment. In the end of composting, similar removal ratio (29.71–99.79%) of ARGs was achieved in different treatments (p greater than 0.05); Chloroflexi became the main phylum and it was closely associated with ARGs removal based on the network analysis. Potential host bacteria of ARGs varied with different antibiotics; in particular, the presence of multiple antibiotics increased potential host bacteria of ermA, sul1 and tetO. Above all, collective effects of different antibiotics led to the enrichment of antibiotic resistance in the composting.

  https://www.sciencedirect.com/science/article/abs/pii/S0960852420310920