摘要

   高级氧化工艺 (AOP) 已被应用于解决多种环境问题，包括抗生素抗性基因 (ARG)。 ARGs 对人类健康的威胁越来越大，它们要么被抗生素抗性细菌 (ARB) 藏匿，要么游离于环境中。然而，ARGs 的控制受到其低浓度和对其界面行为的有限了解的极大限制。在此，设计了一种新型 AOP 催化剂 Ag/TiO2/氧化石墨烯 (GO) 与聚偏二氟乙烯 (PVDF) 超滤膜相结合，具有协同界面吸附和氧化功能，可在模型溶液和二次反应中高效灭活 ARG。废水，特别是当残留物浓度较低时。进一步分析表明，碱基和磷酸二酯的矿化主要引起了 ARGs 的失活。此外，在分子水平上研究了 ARGs 的界面吸附和氧化过程。结果表明，GO富含sp2骨架和功能性氧基团，通过π-π相互作用和氢键有效捕获和富集ARG。因此，光生活性氧物质通过部分克服该过程中的动力学问题来攻击 ARG。 Ag/TiO2/GO 催化剂进一步与 PVDF 膜结合，以测试其在废水处理应用中的潜力。这项工作为细胞内/外 ARGs 的失活和矿化提供了一种有效的方法和相应的材料。此外，对固液界面上 ARG 行为的分子级理解将激发未来 ARG 的进一步控制策略。

  
Advanced oxidation processes (AOPs) have been applied to address multiple environmental concerns including antibiotic resistance genes (ARGs). ARGs have shown an increasing threat to human health, and they are either harbored by antibiotic-resistant bacteria (ARB) or free in the environment. However, the control of ARGs has been substantially limited by their low concentration and the limited knowledge about their interfacial behavior. Herein, a novel AOP catalyst, Ag/TiO2/graphene oxide (GO), combined with a polyvinylidene fluoride (PVDF) ultrafiltration membrane was designed with a synergistic interfacial adsorption and oxidation function to inactivate ARGs with high efficiency in both model solutions and in secondary wastewater effluent, especially when the residue concentration was low. Further analysis showed that the mineralization of bases and phosphodiesters mainly caused the inactivation of ARGs. Moreover, the interfacial adsorption and oxidation processes of ARGs were studied at the molecular level. The results showed that GO was rich in sp2 backbones and functional oxygen groups, which efficiently captured and enriched the ARGs via π-π interactions and hydrogen bonds. Therefore, the photogenerated active oxygen species attack the ARGs by partially overcoming the kinetic problems in this process. The Ag/TiO2/GO catalyst was further combined with a PVDF membrane to test its potential in wastewater treatment applications. This work offers an efficient method and a corresponding material for the inactivation and mineralization of intra/extracellular ARGs. Moreover, the molecular-level understanding of ARG behaviors on a solid–liquid interface will inspire further control strategies of ARGs in the future.

  https://www.sciencedirect.com/science/article/abs/pii/S2095927320304722